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The fluorescence was significantly amplified when pranoprofen was encapsulated within the hydrophobic cavity of \u03b2\u2010cyclodextrin, which provided a favorable microenvironment that stabilized its protonated form. The enhanced fluorescence intensity was measured at 404\u2009nm following excitation at 273\u2009nm with a large Stokes shift of around 130\u2009nm. The method was validated following ICH guidelines and demonstrated excellent linearity over a concentration range of 0.10\u20136.0\u2009\u03bcg\/mL, with a correlation coefficient of 0.9999 and a low limit of detection (LOD) of 0.034\u2009\u03bcg\/mL. The proposed method was effectively applied for the analysis of pranoprofen in ophthalmic solutions and aqueous humor, yielding recoveries ranging from 98.9 to 101.1%. Furthermore, multifaceted sustainability assessment tools confirmed the method's eco\u2010sustainability. This analytical strategy offers a reliable, rapid, and eco\u2010friendly solution for the routine analysis of pranoprofen in pharmaceutical and clinical analysis.<\/jats:p>","DOI":"10.1002\/bio.70461","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:03:36Z","timestamp":1772985816000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unlocking Pranoprofen's Hidden Glow: \u03b2\u2010Cyclodextrin\u2010Driven Fluorescence Amplification of Pranoprofen for Pharmaceutical and Biological Analysis"],"prefix":"10.1002","volume":"41","author":[{"given":"Aya\u00a0Magdy","family":"Saad","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny\u00a0Jeehan\u00a0Mohamed","family":"Nasr","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8153-9697","authenticated-orcid":false,"given":"Asmaa\u00a0Kamal","family":"El\u2010Deen","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Mansoura University  Mansoura Egypt"},{"name":"Department of Agro\u2010Environmental Sciences, Faculty of Agriculture Kyushu University  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,6]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"9","article-title":"Effects of Pranoprofen on Aqueous Humor Monocyte Chemoattractant Protein\u20101 Level and Pain Relief During Second\u2010Eye Cataract Surgery","volume":"9","author":"Zhang Y.","year":"2018","journal-title":"Frontiers in Pharmacology"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcp.13871"},{"key":"e_1_2_7_4_1","first-page":"375","article-title":"Efficacy and Safety of Short\u2010Term Application of Pranoprofen Eye Drops for Senile Patients With Dry Eyes","volume":"18","author":"Li Y. 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