{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T06:43:23Z","timestamp":1777272203288,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009392","name":"Prince Sattam bin Abdulaziz University","doi-asserted-by":"publisher","award":["PSAU\/2025\/03\/33003"],"award-info":[{"award-number":["PSAU\/2025\/03\/33003"]}],"id":[{"id":"10.13039\/100009392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>An environmentally friendly approach was developed for the precise determination of bumetanide and amiloride, two diuretics with complementary therapeutic applications. The method employs an integrated sugaring\u2010out homogeneous liquid\u2013liquid microextraction (SULLME) technique in conjunction with synchronous fluorescence spectroscopy. This approach eliminates toxic organic solvents typically used in liquid\u2013liquid extraction. The two drugs exhibit distinct synchronous fluorescence signatures at a \u0394\u03bb of 140\u2009nm, facilitating baseline\u2010resolved signals and thereby reducing analysis time and costs. The method was linear for bumetanide (0.05\u20130.5\u2009\u03bcg\/mL) and amiloride (0.04\u20131.0\u2009\u03bcg\/mL). Additionally, it exhibits low limits of detection (LODs) of 0.006 and 0.012\u2009\u03bcg\/mL for bumetanide and amiloride, respectively, demonstrating high sensitivity. The approach was successfully applied for the determination of both drugs in coformulation with a challenging ratio and in spiked human urine. A multifaceted assessment that included performance, technique innovation, and sustainability measures was carried out to determine the approach's overall feasibility. By combining environmentally friendly sample preparation and low reagent usage, the proposed strategy adheres to green analytical chemistry principles while preserving good analytical performance. This study shows how advanced spectroscopic techniques combined with green chemistry concepts may produce high\u2010performing, affordable, and ecologically friendly analytical solutions for pharmaceutical multianalyte monitoring.<\/jats:p>","DOI":"10.1002\/bio.70476","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T13:55:28Z","timestamp":1775656528000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic Sugaring\u2010Out Microextraction Coupled With Synchronous Fluorescence for Multiplexed Diuretic Detection in Pharmaceuticals and Biofluids"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9073-9903","authenticated-orcid":false,"given":"Samah\u00a0Abo","family":"El\u00a0Abass","sequence":"first","affiliation":[{"name":"Pharmaceutical Chemistry Department College of Pharmacy, Prince Sattam Bin\u2010Abdul Aziz University  Al\u2010Kharj Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manal\u00a0A.","family":"Alossaimi","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department College of Pharmacy, Prince Sattam Bin\u2010Abdul Aziz University  Al\u2010Kharj Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulmalik\u00a0S.\u00a0A.","family":"Altamimi","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department College of Pharmacy, Prince Sattam Bin\u2010Abdul Aziz University  Al\u2010Kharj Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8153-9697","authenticated-orcid":false,"given":"Asmaa\u00a0Kamal","family":"El\u2010Deen","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department Faculty of Pharmacy, Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12237226"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jch.13413"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ggi.13743"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/155111"},{"key":"e_1_2_10_6_1","first-page":"65","article-title":"Bumetanide\/Amiloride or Frusemide\/Amiloride in Congestive Cardiac Failure in General Practice?","volume":"50","author":"Bremner A. D.","year":"1991","journal-title":"Current Therapeutic Research, Clinical and Experimental"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1185\/03007999009111496"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0003\u20102670(98)00516\u20109"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1556\/achrom.27.2015.3.5"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/37.4.93"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CHROMA.2007.10.026"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2022.116530"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202100255"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio\u20102021\u20100219"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065\u2010023\u201001080\u20104"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2020.122069"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2017.02.051"},{"key":"e_1_2_10_18_1","unstructured":"P.Heney \u201cWhat Is Fluorescence Spectroscopy? \u201d RD World (2023) https:\/\/www.rdworldonline.com\/what\u2010is\u2010fluorescence\u2010spectroscopy\/."},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2023.101321"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2020.120944"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2024.111301"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.4772"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talo.2025.100447"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201026827\u20102"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.4586"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2023.123029"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2025.107586"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2010.04.010"},{"key":"e_1_2_10_29_1","unstructured":"\u201cICH Harmonised Tripartite Guideline Validation of Analytical Procedures: Text and Methodology Q2(R1) \u201d Guideline on Validation of Analytical Procedures: Methodology Developed to Complement the Parent Guideline (2005)."},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c01887"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/analytica5030030"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2025.102051"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2018.01.013"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065\u2010025\u201001486\u20102"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1039\/d3gc02347h"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sampre.2025.100164"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/d4gc05298f"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c00212"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2025.102294"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2025.114781"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1039\/d5ay00015g"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2012.03.018"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2019.03.006"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2010.10.009"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2003.09.002"}],"container-title":["Luminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.70476","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bio.70476","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.70476","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T05:49:21Z","timestamp":1777268961000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bio.70476"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":44,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/bio.70476"],"URL":"https:\/\/doi.org\/10.1002\/bio.70476","archive":["Portico"],"relation":{},"ISSN":["1522-7235","1522-7243"],"issn-type":[{"value":"1522-7235","type":"print"},{"value":"1522-7243","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2025-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70476"}}