{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T08:01:46Z","timestamp":1785225706497,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Epinastine used in treatment and management of allergic conjunctivitis. The development of a simple, sensitive, and cost\u2010effective analytical method for its quantification is essential for pharmaceutical quality control and study. Green (eco\u2010friendly) spectrofluorimetric method for the determination of epinastine in ophthalmic preparations and aqueous humor samples was developed. The method involves converting the cited drug into a fluorescent derivative through a one\u2010pot condensation reaction with fluorescamine, which serves as a detection probe. Fluorescamine is a reagent that reacts specifically with the primary amine moiety of epinastine, forming a highly fluorescent product. In borate buffer (pH\u20098.5), the reaction yielded a fluorescent product with maximum emission intensity at 483\u2009nm upon excitation at 390\u2009nm. The fluorescence intensity showed a linear response within the concentration ranges of 60\u2013400\u2009ng\/mL and 65\u2013350.0\u2009ng\/mL for standard epinastine and spiked aqueous humor samples, respectively. The method demonstrated high sensitivity, with limits of detection (LOD) of 19.5 and 20.60\u2009ng\/mL and limits of quantification (LOQ) of 59.11 and 62.50\u2009ng\/mL for standard epinastine and aqueous humor samples, respectively. The greenness profile of the method was evaluated using four smart metric tools.<\/jats:p>","DOI":"10.1002\/bio.70565","type":"journal-article","created":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T04:49:03Z","timestamp":1783745343000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Reliable Fluorescence Method of One\u2010Pot Condensation for Sensitive Determination of Epinastine in Aqueous Humor: Greenness, Blueness, and Redness Assessment"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6011-1306","authenticated-orcid":false,"given":"Abobakr A.","family":"Mohamed","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Fayoum University  Faiyum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein M.","family":"Elbadawy","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, College of Pharmacy Taibah University  Madinah UAE"},{"name":"Health and Life Research Center Taibah University  Madinah UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohannad A.","family":"Almikhlafi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, College of Pharmacy Taibah University  Madinah UAE"},{"name":"Health and Life Research Center Taibah University  Madinah UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Almaghrabi","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy Taibah University  Al\u2010Madinah Al\u2010Munawwarah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud A. H.","family":"Moustafa","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy Taibah University  Al\u2010Madinah Al\u2010Munawwarah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6635-7606","authenticated-orcid":false,"given":"Mahmoud A.","family":"Omar","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy Taibah University  Al\u2010Madinah Al\u2010Munawwarah Saudi Arabia"},{"name":"Analytical Chemistry Department, Faculty of Pharmacy Minia University  Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,10]]},"reference":[{"issue":"3","key":"e_1_2_9_2_1","first-page":"145","article-title":"Analytical Methods for Isoquinoline Alkaloids: A Review","volume":"8","author":"Smith J. 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