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Herein, an electrochemiluminescence (ECL) sensor for uric acid (UA) detection was constructed via integrating a cascade of bioenzyme (urate oxidase [UOx]) and confined nanozyme reactions. Gold nanoparticles (AuNPs) were confined within amino\u2010functionalized vertical mesoporous silica film (NH\n                    <jats:sub>2<\/jats:sub>\n                    \u2010VMSF) via electrochemical deposition. The NH\n                    <jats:sub>2<\/jats:sub>\n                    \u2010VMSF was synthesized on indium tin oxide (ITO) through electrochemical\u2010assisted self\u2010assembly (EASA) method. Amino groups on the NH\n                    <jats:sub>2<\/jats:sub>\n                    \u2010VMSF surface served as anchoring sites for AuNPs, which were electrochemically reduced and confined within the nanochannels. The confined AuNPs exhibited strong pseudo\u2010peroxidase\u2010like activity, boosting ECL response of luminol\u2010H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    . Compared to the NH\n                    <jats:sub>2<\/jats:sub>\n                    \u2010VMSF\/ITO electrode, the AuNPs\u2010deposited electrode produced a 3.4\u2010fold increase in ECL intensity. Urate oxidase catalyzed the oxidation of uric acid, producing H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    , which acted as a co\u2010reactant for luminol, enabling indirect uric acid detection. The sensor demonstrated a detection range of 0.1\u201320\u2009\u03bcM with a low limit of detection (LOD, 85\u2009nM). The sensor was used for UA detection in complex biological samples (urine). The nanoconfined AuNPs provided a stable and effective environment for nanozyme\u2010catalyzed reactions, ensuring high sensitivity and long\u2010term stability.\n                  <\/jats:p>","DOI":"10.1002\/bio.70592","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T11:42:17Z","timestamp":1784806937000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Electrochemiluminescence Sensor for Uric Acid Detection Using Natural Enzyme\u2010Confined Nanozyme Cascade"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4421-2072","authenticated-orcid":false,"given":"Lanlan","family":"Wu","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering Zhejiang Sci\u2010Tech University  Hangzhou 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