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The reaction culminates in the HRP catalyzed oxidation of Ampliflu Red, generating an intense, quantifiable emission centered at \u03bb\n                    <jats:sub>em<\/jats:sub>\n                    \u2009=\u2009585\u2009nm and excitation at \u03bb\n                    <jats:sub>ex<\/jats:sub>\n                    \u2009=\u2009570\u2009nm, whose intensity is stoichiometrically correlated with cholesterol concentration (\n                    <jats:italic>c<\/jats:italic>\n                    ). Crucially, the inner filter effect, which typically imposes nonlinearity at high analyte concentrations, is mathematically deconvoluted and corrected. This computational approach drastically expands the linear dynamic range from 0.2\u2009mg\/dL to unprecedented 975\u2009mg\/dL, demonstrating a paradigm shift in overcoming fundamental limitations (encompassing concentrations beyond the typical &gt;\u2009375\u2009mg\/dL inflection point) and extending the analytical applicability of fluorescence biosensors into previously inaccessible high\u2010concentration regimes.\n                  <\/jats:p>","DOI":"10.1002\/bio.70608","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T01:18:55Z","timestamp":1787620735000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deconvolution and Correction of the Inner Filter Effect in a Tri\u2010Enzymatic Fluorescent Cascade for Ultra\u2010Extended Cholesterol Quantification"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0210-2647","authenticated-orcid":false,"given":"Surendra K.","family":"Yadav","sequence":"first","affiliation":[{"name":"Department of Chemistry Institute of Science, Banaras Hindu University  Varanasi Uttar Pradesh India"},{"name":"Centre for Nano Science and Engineering Indian Institute of Science  Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1993.1020"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2025.127860"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-7061(98)00178-2"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.08.042"},{"issue":"1","key":"e_1_2_10_6_1","article-title":"Rate of the Liebermann\u2010Burchard Cholesterol Color Reaction","volume":"5","author":"Koehler A. 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