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In this study, uniform spherical silica\u2010encapsulated silicon\u2010nitrogen co\u2010doped carbon dots (Si\u2010MPD\u2010CDs@SiO\n                    <jats:sub>2<\/jats:sub>\n                    ) with a particle size of 24\u2009\u00b1\u200915\u2009nm were prepared via a modified St\u00f6ber method. The nanocomposites were further carboxylated and covalently conjugated with Protein A to construct Si\u2010MPD\u2010CDs@SiO\n                    <jats:sub>2<\/jats:sub>\n                    @PA bioconjugates. The resulting nanoprobe was successfully applied for specific immunofluorescence labeling and imaging of immortalized mouse thymic epithelial cells (iTECs). Compared with commercial fluorescent secondary antibodies, Si\u2010MPD\u2010CDs@SiO\n                    <jats:sub>2<\/jats:sub>\n                    @PA exhibit multiple superiorities including low cost, facile preparation and purification, favorable surface modifiability, strong fluorescence emission, and high photostability. These results indicate that Si\u2010MPD\u2010CDs@SiO\n                    <jats:sub>2<\/jats:sub>\n                    @PA can serve as a promising fluorescent nanoprobe for targeted cellular imaging and related biomedical applications.\n                  <\/jats:p>","DOI":"10.1002\/bio.70627","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T08:13:12Z","timestamp":1790237592000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fabrication of Protein A\u2013Functionalized Silica\u2010Encapsulated Carbon Dot Bioconjugates for Cellular Fluorescence Imaging"],"prefix":"10.1002","volume":"41","author":[{"given":"Yuheng","family":"Wang","sequence":"first","affiliation":[{"name":"College of Basic Medical Science, Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Yichang Key Laboratory of Infection and Inflammation China Three Gorges University  Yichang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihong","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Basic Medical Science, Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Yichang Key Laboratory of Infection and Inflammation China Three Gorges University  Yichang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxin","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Basic Medical Science, Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Yichang Key Laboratory of Infection and Inflammation China Three Gorges University  Yichang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changwen","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Basic Medical Science, Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Yichang Key Laboratory of Infection and Inflammation China Three Gorges University  Yichang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Basic Medical Science, Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Yichang Key Laboratory of Infection and Inflammation China Three Gorges University  Yichang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"College of Basic Medical Science, Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Yichang Key Laboratory of Infection and Inflammation China Three Gorges University  Yichang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2000-3167","authenticated-orcid":false,"given":"Yongqin","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Basic Medical Science, Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Yichang Key Laboratory of Infection and Inflammation China Three Gorges University  Yichang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5745-6613","authenticated-orcid":false,"given":"Jiaxuan","family":"Han","sequence":"additional","affiliation":[{"name":"College of Basic Medical Science, Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Yichang Key Laboratory of Infection and Inflammation China Three Gorges University  Yichang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyun","family":"Liu","sequence":"additional","affiliation":[{"name":"Hubei Longsheng Taike Medical Devices Co. 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