{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T06:37:29Z","timestamp":1793083049280,"version":"4.5.0"},"reference-count":64,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A novel direct spectrofluorimetric method was developed and validated for simultaneous determination of cetirizine hydrochloride (CTZ) and montelukast sodium (MKT), commonly used in COVID\u201019 combination therapy. Montelukast was determined using first derivative synchronous fluorescence at 422\u2009nm, corresponding to a zero\u2010crossing point for CTZ. Cetirizine was quantified using a newly applied ratio derivative synchronous fluorescence spectroscopy (\n                    <jats:sup>1<\/jats:sup>\n                    DD\u2010SFS) technique, using MKT (0.1\u2009\u03bcg\/mL) as a divisor, allowing CTZ measurement at 293\u2009nm. Cetirizine and montelukast native fluorescence was significantly enhanced by micellar solubilization using cetyltrimethylammonium bromide (CTAB) and sulfuric acid. A two\u2010level factorial design was employed for method optimization, with optimal conditions incorporating 0.5%\n                    <jats:italic>w\/v<\/jats:italic>\n                    CTAB and 0.2\u2010M sulfuric acid. Validation was performed according to ICH guidelines, proving the methods specificity, sensitivity, and robustness. The methods demonstrated linearity in the range of 0.1\u20131.0\u2009\u03bcg\/mL for MKT and 1.0\u201310.0\u2009\u03bcg\/mL for CTZ, with detection limits of 0.017 and 0.415\u2009\u03bcg\/mL, respectively. The developed methods were applied for MKT and CTZ concurrent determination in laboratory\u2010prepared mixtures simulating tablet formulations. Greenness and practicality were assessed using Eco\u2010Scale, the Complex MoGAPI, AGREE, and BAGI establishing the method as eco\u2010friendly and suitable for routine quality control.\n                  <\/jats:p>","DOI":"10.1002\/bio.70632","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:57:52Z","timestamp":1790409472000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Sustainable, Quality\u2010by\u2010Design (QbD) Inspired Spectrofluorimetric Method for the Concurrent Analysis of Cetirizine and Montelukast in COVID\u201019 and Allergic Rhinitis Combination Therapy"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9571-0771","authenticated-orcid":false,"given":"Basma Z.","family":"El\u2010Khateeb","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy University of Tanta  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5548-8742","authenticated-orcid":false,"given":"Mona M.","family":"Amer","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy University of Tanta  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary A.","family":"Michael","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy University of Tanta  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,26]]},"reference":[{"key":"e_1_2_14_2_1","volume-title":"USP Monograph for Montelukast Sodium","author":"The United States Pharmacopeial Convention","year":"2019"},{"key":"e_1_2_14_3_1","volume-title":"USP Monograph for Certirizine","author":"United States Pharmacopeial Convention","year":"2020"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.108412"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/02770903.2024.2415544"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2020.110046"},{"key":"e_1_2_14_7_1","first-page":"450","article-title":"Validated UV Spectroscopic Method for Estimation of Montelukast Sodium From Bulk and Tablet Formulations","volume":"1","author":"Pallavi K.","year":"2012","journal-title":"International Journal of Advances in Pharmacy, Biology and Chemistry"},{"key":"e_1_2_14_8_1","first-page":"482","article-title":"Validated UV Spectroscopic Method for Estimation of Montelukast Sodium From Bulk and Tablet Formulations","volume":"4","author":"Chaudhary M.","year":"2018","journal-title":"International Journal of Pharma and Bio Sciences"},{"key":"e_1_2_14_9_1","first-page":"382","article-title":"UV\u2010Visible Spectrophotometric Estimation of Montelukast and Fexofenadine by Simultaneous Equation Method in Bulk & Combined Tablet Dosage Form","volume":"11","author":"Patle D.","year":"2017","journal-title":"Current Trends in Biotechnology and Pharmacy"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065-024-01116-3"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.211196"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.201156"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2022.107236"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.21276\/apjhs.2022.9.3.49"},{"key":"e_1_2_14_15_1","first-page":"61","article-title":"Development and Validation of a Stability Indicating RP\u2010UPLC Method for Simultaneous Determination of Rupatadine and Montelukast in Pharmaceutical Formulation","volume":"9","author":"Kumar N.","year":"2013","journal-title":"Current Pharmaceutical Analysis"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5540907"},{"key":"e_1_2_14_17_1","first-page":"502","article-title":"Potentiometric Method to Determine Montelukast Sodium in Its Tablets With In\u2010Line Monitoring of Its Dissolution Behaviour","volume":"12","author":"Soudi A. 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