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In this work, a small\u2010molecule fluorescent probe was designed for the selective recognition of CBZ. The extended\n                    <jats:italic>\u03c0<\/jats:italic>\n                    \u2010conjugated plane and heteroatom\u2010containing sites of the probe were designed to match the fused aromatic framework and amide\/carbonyl features of CBZ. This molecular\u2010level complementarity may facilitate noncovalent probe\u2013CBZ association and contribute to the observed fluorescence quenching response. Its sensing properties, stability, resistance to interference, biocompatibility, and fluorescence response mechanism were then comprehensively evaluated. The probe showed a CBZ concentration\u2010dependent fluorescence quenching behavior within the range of 0\u20131000\u2009\u03bcg\/mL, and the limit of detection was calculated to be 2.351\u2009\u03bcg\/mL. Stable fluorescence emission was retained under pH\u20096\u20139, and the probe also exhibited favorable photostability. Zebrafish embryo experiments showed no obvious influence, indicating low biological toxicity and good environmental compatibility. Mechanistic investigations involving XPS, FTIR, DFT calculations, fluorescence lifetime analysis, Stern\u2013Volmer analysis, and DLS collectively support a noncovalent probe\u2013CBZ association process accompanied by electronic redistribution and changes in the association state. Taken together, the developed probe provides a preliminary fluorescence\u2010based strategy for CBZ analysis in aqueous systems.\n                  <\/jats:p>","DOI":"10.1002\/bio.70635","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:01:13Z","timestamp":1791180073000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Small\u2010Molecule Fluorescent Probe for Rapid and Selective Detection of Carbamazepine in Aquatic Environments"],"prefix":"10.1002","volume":"41","author":[{"given":"Zhenzhen","family":"Liu","sequence":"first","affiliation":[{"name":"Fuzhou First General Hospital Affiliated With Fujian Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhicen","family":"Qiu","sequence":"additional","affiliation":[{"name":"School of Ecological Environment and Urban Construction Fujian University of Technology  Fuzhou 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