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Herein, an electrochemiluminescence (ECL) immunosensor was constructed based on the synergistic effect of spatial confinement\u2010enhanced catalysis and immunorecognition gating by confining nitrogen\u2010doped graphene quantum dots (NGQDs) within vertically ordered mesoporous silica nanochannel film (VMSF) through electrophoresis. NGQDs exhibit peroxidase\u2010like activity, efficiently catalyzing reactive oxygen species (ROS) generation from H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    and amplifying the ECL signal. Following epoxy group functionalization on VMSF's outer surface, PSA antibodies were covalently immobilized to construct a recognitive interface. Specific binding of PSA to antibody forms immunocomplex that introduces steric hindrance, hindering mass transfer of luminol and H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    and increasing interface resistance. This causes ECL signal decrease, enabling the quantitative determination of PSA. The fabricated immunosensor can linearly detect PSA from 0.01 to 100\u2009ng\u2009mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    with limit of detection (LOD) of 1.9\u2009pg\u2009mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    . The immunosensor also demonstrated good anti\u2010interference capability, high stability, and reproducibility. PSA detection in human serum samples was realized. The strategy for the fabrication of immunosensor integrated confinement\u2010enhanced catalysis and gated immunorecognition, showing advantages including simple fabrication, controllable confinement, and no need for antibody label.\n                  <\/jats:p>","DOI":"10.1002\/bio.70636","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:30:02Z","timestamp":1791261002000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Electrochemiluminescence Immunosensor Based on Confinement\u2010Enhanced Catalysis and Immunorecognition Gating for Sensitive Determination of Prostate\u2010Specific Antigen"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6629-8065","authenticated-orcid":false,"given":"Jiaqi","family":"Peng","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering Zhejiang Sci\u2010Tech University  Hangzhou 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