{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T06:37:33Z","timestamp":1793083053920,"version":"4.5.0"},"reference-count":28,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mirogabalin (MGB) is a new gabapentinoid drug and is designated for peripheral neuropathic pain, involving peripheral neuropathic pain related to diabetes and postherpetic neuralgia. Despite its critical therapeutic importance, reported methods for its determination remain scarce and mainly dependent on LC\u2013MS\/MS methods. These published methods demand costly equipment and lengthy sample preparation. Herein, we describe the development and validation of the first spectrofluorimetric report for the sensitive assay of MGB in bulk drug, pharmaceutical tablets, and spiked human urine and plasma. The quantification strategy relies on the Hantzsch condensation reaction between amino group of MGB with acetylacetone and formaldehyde under mildly acidic conditions to produce a strongly fluorescent product. The reaction product was confirmed using\n                    <jats:sup>1<\/jats:sup>\n                    H NMR and\n                    <jats:sup>13<\/jats:sup>\n                    C NMR analysis. Calibration was linear across 0.1\u20132.0\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL in the pure drug standard, with a detection limit of 0.029\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL and a quantification limit of 0.089\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL. The linear ranges for spiked human urine and plasma are 0.2\u20132.0 and 0.1\u20131.5, respectively. Application to spiked human urine and plasma yielded mean recoveries of 98.28\u2009\u00b1\u20091.14\u201398.74\u2009\u00b1\u20090.91%. Moreover, the introduced was method used for investigating the content uniformity testing of the drug tablets.\n                  <\/jats:p>","DOI":"10.1002\/bio.70643","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:23:01Z","timestamp":1791264181000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First Spectrofluorimetric Determination of Mirogabalin in Biological Fluids: A Validated Hantzsch Derivatization Strategy With Full NMR Structural Confirmation"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6011-1306","authenticated-orcid":false,"given":"Abobakr A.","family":"Mohamed","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Fayoum University  Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5469-0129","authenticated-orcid":false,"given":"Mostafa","family":"Sayed","sequence":"additional","affiliation":[{"name":"School of Materials and Chemistry Southwest University of Science and Technology  Mianyang China"},{"name":"Department of Chemistry, Faculty of Science New Valley University  El\u2010Kharja Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3703-7221","authenticated-orcid":false,"given":"Islam M.","family":"Mostafa","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Minia University  Minia Egypt"},{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Minia National University  New Minia Egypt"},{"name":"Institute of Solid State Physics Hefei Institutes of Physical Science, Chinese Academy of Sciences  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurol.2018.09.016"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2015.01.018"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000001939"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(10)70143-5"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000492"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdi.13013"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40122-024-00617-2"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/14656566.2021.1958780"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2021.01.002"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1358\/dot.2020.56.2.3100504"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1254\/fpj.154.352"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40261-018-0692-7"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/prp2.418"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12325-022-02424-7"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/00498254.2021.1879408"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11419-026-00766-3"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2025.344446"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.4570"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.23.413"},{"key":"e_1_2_9_21_1","first-page":"111","article-title":"Spectrophotometric and Spectrofluorimetric Determination of Amlodipine Besilate and Doxazosin Mesilate in Bulk and in Dosage Forms via Hantzsch Reaction","volume":"3","author":"Ayad M.","year":"2012","journal-title":"International Journal of Pharmaceutical and Biomedical Research"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.2174\/0113852728331961240918115757"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejoc.201901923"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2018.05.009"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.4178"},{"key":"e_1_2_9_26_1","unstructured":"ICH \u201cValidation of Analytical Procedures: Text and Methodology ICH Harmonised Tripartite Guideline Q2(R1) \u201d (2005)."},{"key":"e_1_2_9_27_1","first-page":"161","volume-title":"Handbook of Modern Pharmaceutical Analysis","author":"Sheinin E. 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