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In addition, CdTe quantum dot (QD)\u2010labelled microspheres, comprising polystyrene (PS) cores and CdTe\/polyelectrolyte (PE) shells, were also prepared by the layer\u2010by\u2010layer technique in this paper. The optical properties of the CdTe nanoparticles and CdTe\u2010labelled microspheres were investigated by UV\u2010Visible absorption and luminescence spectroscopy, and fluorescence microscopy was employed for microscopic identification behaviour of the luminescent microspheres. 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