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A digoxin hapten was indirectly heavily labelled with Ru(bpy)<jats:sub>2<\/jats:sub>(dcbpy)NHS through BSA to form Ru(bpy)<jats:sub>2<\/jats:sub>(dcbpy)NHS\u2013BSA\u2013digoxin conjugate. The ECL intensity of the immunocomplex of the conjugate with anti\u2010digoxin antibody markedly decreased when the immunoreaction between Ru(bpy)<jats:sub>2<\/jats:sub>(dcbpy)NHS\u2013BSA\u2013digoxin conjugate and anti\u2010digoxin antibody took place. Two formats, direct homogeneous immunoassay for anti\u2010digoxin antibody and competitive immunoassay for digoxin, were developed to determine anti\u2010digoxin antibody and digoxin, respectively. The anti\u2010digoxin antibody concentration in the range 7.6 \u00d7 10<jats:sup>\u22128<\/jats:sup>\u20137.6 \u00d7 10<jats:sup>\u22126<\/jats:sup> g\/mL was determined by direct homogeneous format. Digoxin hapten was determined throughout the range 4.0 \u00d7 10<jats:sup>\u221210<\/jats:sup>\u20131.0 \u00d7 10<jats:sup>\u22127<\/jats:sup> g\/mL with a detection limit of 1.0 \u00d7 10<jats:sup>\u221210<\/jats:sup> g\/mL by competitive format. The relative standard derivation for 6.0 \u00d7 10<jats:sup>\u22129<\/jats:sup> g\/mL was 4.3%. The method has been applied to assaying digoxin in control human serum. 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