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In this study, we examined effect of LPA on prostaglandin (PG) E<jats:sub>2<\/jats:sub>production in MKN74 cells, a gastric cell\u2010line expressing type 2 LPA receptor (LPA2). When the cells were treated with LPA, the level of mRNA of COX\u20102 but not COX\u20101 was upregulated. The LPA effect was abolished when the cells were pretreated with pertussis toxin (PTX), suggesting the involvement of receptor(s) coupled with Gi. Pretreatment of MKN74 cells with LPA enhanced the PGE<jats:sub>2<\/jats:sub>production triggered by calcium ionophore A23187. Again, PTX abolished the LPA effect. Fluorescent immunohistochemistry using an antibody against LPA2 showed that surface mucous cells (pit cells) in gastric mucosa of mice express LPA2 on the apical side of the plasma membrane. These results suggest that LPA in the diet or its digestion may contribute to the epithelial integrity of stomach mucosa by enhancement of PGE<jats:sub>2<\/jats:sub>production via activation of LPA2. \u00a9 2013 BioFactors, 40(3):355\u2013361, 2014<\/jats:p>","DOI":"10.1002\/biof.1147","type":"journal-article","created":{"date-parts":[[2013,12,21]],"date-time":"2013-12-21T06:51:21Z","timestamp":1387608681000},"page":"355-361","source":"Crossref","is-referenced-by-count":9,"title":["Type 2 lysophosphatidic acid receptor in gastric surface mucous cells: Possible implication of prostaglandin E<sub>2<\/sub>production"],"prefix":"10.1002","volume":"40","author":[{"given":"Tamotsu","family":"Tanaka","sequence":"first","affiliation":[{"name":"Institute of Health Biosciences, University of Tokushima Graduate School Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayumi","family":"Ohmoto","sequence":"additional","affiliation":[{"name":"Institute of Health Biosciences, University of Tokushima Graduate School Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuya","family":"Morito","sequence":"additional","affiliation":[{"name":"Institute of Health Biosciences, University of Tokushima Graduate School Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Science Fukuyama University Fukuyama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mai","family":"Urikura","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Science Fukuyama University Fukuyama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoshi","family":"Satouchi","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Science Fukuyama University Fukuyama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Tokumura","sequence":"additional","affiliation":[{"name":"Institute of Health Biosciences, University of Tokushima Graduate School Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,12,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20081"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2010.04.015"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1248\/jhs.57.115"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2012.08.020"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1999.0029900368"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.34209"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.03.038"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00231.2011"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050421"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00345.2009"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00172.2011"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-011-1595-0"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-012-2475-y"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00004.2008"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2005.06.004"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.10R06CR"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1016\/S0022-3565(24)29646-4","article-title":"Adaptive gastric cytoprotection is mediated by prostaglandin EP1 receptors: a study using rats and knockout mice","volume":"297","author":"Takeuchi K.","year":"2001","journal-title":"J. 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