{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T03:17:49Z","timestamp":1789615069951,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,8,11]],"date-time":"2014-08-11T00:00:00Z","timestamp":1407715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[2014,9,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Obesity induced metabolic syndrome is increasing worldwide at an alarming rate. It is characterized by excessive expansion of white adipose tissue which leads to hypoxia and impairs normal metabolism. Recent studies reveal that hypoxia could be one of the factors for inflammation, insulin resistance and other obesity related complications. There is a high demand for anti\u2010obese phytoceuticals to control and manage the complications resulting from obesity. In this study, we investigated how hypoxia affect the physiological functions of 3T3\u2010L1 adipocytes emphasizing on oxidative stress, inflammation, and mitochondrial functions. We also evaluated the protective role of various doses of curcumin, a well\u2010known dietary antioxidant, on hypoxia induced alterations. The results revealed that hypoxia significantly altered the vital parameters of adipocyte biology like HIF 1\u03b1 expression (103.47% \u2191), lactate, and glycerol release (184.34% and 69.1% \u2191, respectively), reactive oxygen species production (432.53% \u2191), lipid and protein oxidation (376.6% and 566.6% \u2191, respectively), reduction in antioxidant enzymes (superoxide dismutase and catalase) status, secretion of inflammatory markers (TNF \u03b1, IL 6, IL 1\u03b2, and IFN \u03b3), and mitochondrial functions (mitochondrial mass, membrane potential, permeability transition pore integrity, and superoxide generation). Curcumin substantially protected adipocytes from toxic effects of hypoxia in a dose dependent manner by protecting mitochondria and down regulating inflammation. Acriflavine is used as a positive control. A detailed investigation is required for the development of curcumin as an effective nutraceutical against obesity. \u00a9 2014 BioFactors, 40(5):513\u2013523, 2014<\/jats:p>","DOI":"10.1002\/biof.1175","type":"journal-article","created":{"date-parts":[[2014,8,11]],"date-time":"2014-08-11T12:35:52Z","timestamp":1407760552000},"page":"513-523","source":"Crossref","is-referenced-by-count":31,"title":["Curcumin improves hypoxia induced dysfunctions in 3T3\u2010L1 adipocytes by protecting mitochondria and down regulating inflammation"],"prefix":"10.1002","volume":"40","author":[{"given":"Ariyapalli","family":"Priyanka","sequence":"first","affiliation":[{"name":"Agroprocessing and Natural Products Division Council of Scientific and Industrial Research\u2010National Institute for Interdisciplinary Science and Technology (CSIR\u2010NIIST) Thiruvananthapuram 695019 Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasidharan Suseela","family":"Anusree","sequence":"additional","affiliation":[{"name":"Agroprocessing and Natural Products Division Council of Scientific and Industrial Research\u2010National Institute for Interdisciplinary Science and Technology (CSIR\u2010NIIST) Thiruvananthapuram 695019 Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijayakumar Marykutty","family":"Nisha","sequence":"additional","affiliation":[{"name":"Agroprocessing and Natural Products Division Council of Scientific and Industrial Research\u2010National Institute for Interdisciplinary Science and Technology (CSIR\u2010NIIST) Thiruvananthapuram 695019 Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kozhiparambil Gopalan","family":"Raghu","sequence":"additional","affiliation":[{"name":"Agroprocessing and Natural Products Division Council of Scientific and Industrial Research\u2010National Institute for Interdisciplinary Science and Technology (CSIR\u2010NIIST) Thiruvananthapuram 695019 Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(02)00849-5"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200319246"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN20041213"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00435.2007"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2337\/db06-0911"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-007-0301-8"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2007.06.005"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00192-09"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00435.2007"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1079\/PNS2004406"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/13813450802306602"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2337\/db08-0457"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665109990206"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.010"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.07.005"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.01.039"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.135509"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.12.154"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028784"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.2010.00341.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.426338"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1968.tb00428.x"},{"key":"e_1_2_6_24_1","first-page":"130","article-title":"A modified spectrophotometric assay of superoxide dismutase","volume":"21","author":"Kakkar P.","year":"1984","journal-title":"Indian J. Biochem. Biophys."},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(70)90083-7"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/35007508"},{"key":"e_1_2_6_27_1","first-page":"228","article-title":"Adipocyte blood flow is decreased in obese Zucker rats","volume":"3","author":"West D. B.","year":"1987","journal-title":"Am. J. Physiol."},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00017.2012"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-011-2103-y"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.2.5804.25"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-011-2191-8"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-009-0750-3"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-8174com"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00557.2001"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijo.0802407"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90760.2008"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI21625"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.01.004"},{"key":"e_1_2_6_39_1","first-page":"4507","article-title":"Cerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia\u2010induced oxidative stress and inflammation","volume":"8","author":"Arya A.","year":"2013","journal-title":"Int. J. Nanomed."},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0692"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOL.0b013e3282f39f95"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI21752"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-006-0170-2"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(95)00003-A"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0b013e3182756ad3"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2013.02.014"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.106.025817"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-7075-5-17"}],"container-title":["BioFactors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiof.1175","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiof.1175","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biof.1175","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T22:38:17Z","timestamp":1696631897000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/biof.1175"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,11]]},"references-count":47,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,9,10]]}},"alternative-id":["10.1002\/biof.1175"],"URL":"https:\/\/doi.org\/10.1002\/biof.1175","archive":["Portico"],"relation":{},"ISSN":["0951-6433","1872-8081"],"issn-type":[{"value":"0951-6433","type":"print"},{"value":"1872-8081","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,11]]}}}