{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T17:05:42Z","timestamp":1765040742929,"version":"3.37.3"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,10,19]],"date-time":"2017-10-19T00:00:00Z","timestamp":1508371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A double\u2010blind, randomized, placebo\u2010controlled clinical trial assessed the efficacy and safety of <jats:italic>Ageratum conyzoides<\/jats:italic> in treating benign prostatic hypertrophy (BPH). In this study, 109 men with medically diagnosed BPH, aged 41\u201376 years, were administered the investigational product, <jats:italic>A. conyzoides<\/jats:italic> extract at a dose of 250 mg\/d or placebo, q.d. for 12 weeks. The primary outcome measures were the International Prostate Symptom Score (IPSS), daily urinary frequency and safety evaluations. The secondary outcome measures were testosterone, dihydrotestosterone, oestradiol, sex hormone binding globulin (SHBG), Dehydroepiandrosterone sulfate (DHEA\u2010S) and cortisol levels, and prostate specific antigen (PSA), lipids, blood glucose, the Aging Male's Symptom (AMS) Score and sexual function assessed by Derogatis Interview for Sexual Functioning\u2010Self Report (DISF\u2010SR). The effect of <jats:italic>A. conyzoides L<\/jats:italic> extract on gene expression of 5\u2010alpha\u2010reductase in human prostate cells was also investigated to elucidate a potential mechanism of action. The clinical study, showed a significant reduction in total IPSS score (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) and day\u2010 and night\u2010time urinary frequency (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) over time after treatment with <jats:italic>A. conyzoides<\/jats:italic>. Steroid hormones, SHBG, PSA levels, lipids, and blood glucose remained within healthy reference range in both groups. There were no changes in AMS or DISF\u2010SR in either group. Gene arrays demonstrated that <jats:italic>A. conyzoides<\/jats:italic> extract was effective in reducing the expression of mRNA coding for 5\u2010alpha\u2010reductase types 2 and 1 in human prostate epithelial cells. The overall results indicate that <jats:italic>A. conyzoides<\/jats:italic> may be an effective treatment for reducing symptoms of BPH in healthy men, in part, through inhibition of 5\u2010alpha\u2010reductase enzyme activity. \u00a9 2017 BioFactors, 43(6):789\u2013800, 2017<\/jats:p>","DOI":"10.1002\/biof.1389","type":"journal-article","created":{"date-parts":[[2017,10,19]],"date-time":"2017-10-19T13:26:37Z","timestamp":1508419597000},"page":"789-800","source":"Crossref","is-referenced-by-count":7,"title":["<i>Ageratum conyzoides<\/i> L. inhibits 5\u2010alpha\u2010reductase gene expression in human prostate cells and reduces symptoms of benign prostatic hypertrophy in otherwise healthy men in a double blind randomized placebo controlled clinical study"],"prefix":"10.1002","volume":"43","author":[{"given":"Matthew","family":"Detering","sequence":"first","affiliation":[{"name":"School of Chemistry Queensland University of Technology Brisbane QLD Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4743-2751","authenticated-orcid":false,"given":"Elizabeth","family":"Steels","sequence":"additional","affiliation":[{"name":"Sydney Medical School The University of Sydney Sydney NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sundar Rao","family":"Koyyalamudi","sequence":"additional","affiliation":[{"name":"Institute of Endocrinology and Diabetes, The Children's Hospital at Westmead Sydney NSW Australia"},{"name":"Discipline of Paediatrics and Child Health, The Children's Hospital at Westmead The University of Sydney Sydney NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Allifranchini","sequence":"additional","affiliation":[{"name":"ABICH S.r.l Biological and chemical analysis, via 42 Martiri 213\/B 28924 Verbania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Bocchietto","sequence":"additional","affiliation":[{"name":"ABICH S.r.l Biological and chemical analysis, via 42 Martiri 213\/B 28924 Verbania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7490-9298","authenticated-orcid":false,"given":"Luis","family":"Vitetta","sequence":"additional","affiliation":[{"name":"Sydney Medical School The University of Sydney Sydney NSW Australia"},{"name":"Medlab Clinical Sydney NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)49698-4"},{"key":"e_1_2_8_3_1","first-page":"S3","article-title":"Pathophysiology of benign prostatic hyperplasia in the aging male population","volume":"7","author":"Lepor H.","year":"2005","journal-title":"Rev. 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