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In this study, Medicarpin (Med), a natural pterocarpan, was selected (by screening) as a small\u2010molecule inducer of adipocyte differentiation among 854 candidates by using C3H10T1\/2 mesenchymal stem cell; a cellular model of adipogenesis. Med induced the expression of brown\u2010adipocyte commitment marker Bmp7 as well as the early regulators of brown fat fate Ppar\u03b3, Prdm16, and Pgc\u20101\u03b1 during differentiation of C3H10T1\/2 mesenchymal stem cells. Med also induced the expression of a key thermogenic marker\u2014uncoupling protein 1 (UCP1)\u2014along with expression of other brown\u2010fat\u2013specific markers and beige\u2010fat\u2013specific markers. Of note, Med significantly reduced the expression of white fat markers too. Furthermore, Med treatment promoted formation of multilocular lipid droplets (LDs), expression of mitochondrial\u2010biogenesis\u2013related genes, and increased oxygen consumption. Gene silencing study revealed that Med promotes the development of brown\u2010 and beige\u2010adipocyte characteristics in C3H10T1\/2 mesenchymal stem cells through activation of the AMPK pathway, and our data allow us to propose Med as a candidate for therapeutics against obesity or related metabolic disorders. \u00a9 2017 BioFactors, 44(2):168\u2013179, 2018<\/jats:p>","DOI":"10.1002\/biof.1392","type":"journal-article","created":{"date-parts":[[2017,10,24]],"date-time":"2017-10-24T12:32:37Z","timestamp":1508848357000},"page":"168-179","source":"Crossref","is-referenced-by-count":32,"title":["A pivotal role of AMPK signaling in medicarpin\u2010mediated formation of brown and beige"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0755-8499","authenticated-orcid":false,"given":"Khan Mohammad","family":"Imran","sequence":"first","affiliation":[{"name":"Institute of Tissue Regeneration, College of Medicine Soonchunhyang University Cheonan Chung\u2010nam, 330\u2010090 Korea"},{"name":"Department of Microbiology, College of Medicine Soonchunhyang University Cheonan Chung\u2010nam, 330\u2010090 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0978-621X","authenticated-orcid":false,"given":"Dahyeon","family":"Yoon","sequence":"additional","affiliation":[{"name":"Institute of Tissue Regeneration, College of Medicine Soonchunhyang University Cheonan Chung\u2010nam, 330\u2010090 Korea"},{"name":"Department of Microbiology, College of Medicine Soonchunhyang University Cheonan Chung\u2010nam, 330\u2010090 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2057-8534","authenticated-orcid":false,"given":"Yong\u2010Sik","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute of Tissue Regeneration, College of Medicine Soonchunhyang University Cheonan Chung\u2010nam, 330\u2010090 Korea"},{"name":"Department of Microbiology, College of Medicine Soonchunhyang University Cheonan Chung\u2010nam, 330\u2010090 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.07.015"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67483-1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","unstructured":"LakdawallaD.&PhilipsonT.(2002)The growth of obesity and technological change: a theoretical and empirical examination. 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