{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T19:54:51Z","timestamp":1772308491547,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T00:00:00Z","timestamp":1572307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Many population studies have shown that blood concentrations of high\u2010density lipoprotein (HDL) cholesterol are inversely correlated with risk of cardiovascular disease (CVD). However, in recent studies, increasing blood HDL cholesterol concentrations failed to reduce CVD events. On the other hand, studies suggest that improving HDL quality can be a more efficient tool for assessing atherosclerotic risk than simply measuring blood HDL cholesterol concentration. Thus, improving HDL activity using natural substances might be a useful therapeutic approach to reducing CVD risk. We previously isolated a novel active compound from <jats:italic>Nannochloropsis<\/jats:italic> microalgae termed lyso\u2010diacylglyceryltrimethylhomoserine (lyso\u2010DGTS), which increased activity of paraoxonase 1, the main antioxidant enzyme associated with HDL. Here we examined the effect of lyso\u2010DGTS on HDL quality and function. Tryptophan\u2010fluorescence\u2010quenching assay showed that lyso\u2010DGTS interacts spontaneously with the entire HDL lipoprotein and with apolipoprotein A1 (ApoA1), the major structural and functional HDL protein, with high affinity (<jats:italic>K<\/jats:italic><jats:sub>a<\/jats:sub> = 2.17\u2009\u00d7\u200910<jats:sup>4<\/jats:sup>\u2009M<jats:sup>\u22121<\/jats:sup> at 37\u00b0C). Lyso\u2010DGTS added to HDL and to ApoA1 increased cholesterol efflux from macrophage cells, the main antiatherogenic function of HDL, dose\u2010dependently, and significantly increased HDL's ability to induce nitric oxide production from endothelial cells. In\u2010vivo supplementation of lyso\u2010DGTS to the circulation of mice fed a high\u2010fat diet via osmotic mini\u2010pumps implanted subcutaneously enhanced HDL anti\u2010inflammatory effect significantly as compared to controls. Our findings suggest that lyso\u2010DGTS may have a beneficial effect in decreasing atherosclerosis risk by interacting with HDL particles and improving their quality and antiatherogenic functions.<\/jats:p>","DOI":"10.1002\/biof.1580","type":"journal-article","created":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T09:49:12Z","timestamp":1572342552000},"page":"146-157","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Lyso\u2010diacylglyceryltrimethylhomoserine (lyso\u2010DGTS) isolated from <i>Nannochloropsis<\/i> microalgae improves high\u2010density lipoprotein (HDL) functions"],"prefix":"10.1002","volume":"46","author":[{"given":"Ali","family":"Khattib","sequence":"first","affiliation":[{"name":"Department of Oxidative Stress and Human Diseases MIGAL\u2010Galilee Research Institute  Kiryat Shmona Israel"},{"name":"Tel\u2010Hai College  Upper Galilee Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dana","family":"Atrahimovich","sequence":"additional","affiliation":[{"name":"Department of Oxidative Stress and Human Diseases MIGAL\u2010Galilee Research Institute  Kiryat Shmona Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loureen","family":"Dahli","sequence":"additional","affiliation":[{"name":"Department of Oxidative Stress and Human Diseases MIGAL\u2010Galilee Research Institute  Kiryat Shmona Israel"},{"name":"Tel\u2010Hai College  Upper Galilee Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob","family":"Vaya","sequence":"additional","affiliation":[{"name":"Department of Oxidative Stress and Human Diseases MIGAL\u2010Galilee Research Institute  Kiryat Shmona Israel"},{"name":"Tel\u2010Hai College  Upper Galilee 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