{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T17:53:32Z","timestamp":1775843612476,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T00:00:00Z","timestamp":1598486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Hunan Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["2017JJ2374"],"award-info":[{"award-number":["2017JJ2374"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Osteoarthritis (OA) represents a progressive degenerative disorder that predominantly affects the synovial membranes of joints. Recent studies have highlighted the significant role played by microRNAs (miRNAs) in OA development. The current study aimed to elucidate the underlying modulatory role of miR\u201027b\u20103p in the development of OA. The expression of miR\u201027b\u20103p in the OA patients and rat models post anterior cruciate ligament transection operation was measured using reverse transcription quantitative polymerase chain reaction, through which overexpressed miR\u201027b\u20103p was found in both of the samples. To further explore the miR\u201027b\u20103p functions in OA, western blot analysis, enzyme\u2010linked immunosorbent assay, and \u03b2\u2010galactosidase activity assay were conducted with the results showing that knockdown of miR\u201027b\u20103p promoted expression of the osteogenic differentiation markers while inhibiting expression of the adipogenic differentiation markers, inflammatory factors, and cellular senescence of bone marrow mesenchymal stem cells (BMSCs). After that, the interactions between miR\u201027b\u20103p, lysine Demethylase 4B (KDM4B), and Distal\u2010Less Homeobox 5 (DLX5) identified using dual\u2010luciferase reporter gene assay and ChIP assay revealed that miR\u201027b\u20103p inhibited KDM4B and further reduced expression of DLX5. Finally, the paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were assessed in rat models, and increased PWT and PWL were detected after miR\u201027b\u20103p silencing. In conclusion, suppression of miR\u201027b\u20103p could enhance KDM4B and DLX5 to alleviate OA pain, shedding light on a new potential therapeutic target for OA.<\/jats:p>","DOI":"10.1002\/biof.1670","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T14:32:55Z","timestamp":1598625175000},"page":"788-802","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Inhibition of <scp>microRNA<\/scp>\u201027b\u20103p relieves osteoarthritis pain via regulation of <scp>KDM4B<\/scp>\u2010dependent <scp>DLX5<\/scp>"],"prefix":"10.1002","volume":"46","author":[{"given":"Guixiang","family":"Zhang","sequence":"first","affiliation":[{"name":"Operating Room Xiangtan Central Hospital  Xiangtan 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