{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T14:18:42Z","timestamp":1790605122346,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T00:00:00Z","timestamp":1606176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771049"],"award-info":[{"award-number":["81771049"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Accompanying with diabetes mellitus\u2010induced osteoporosis (DM\u2010OS), diabetic patients show poor peri\u2010implant osteogenesis after implantation for dentition defect. Berberine (BBR), a candidate oral hypoglycemic agent, is a promising agent for treating DM\u2010OS. In this study, BBR was applied on DM rats and high\u2010glucose\u2010cultured bone mesenchymal stem cells (BMSCs) to investigate its therapeutic mechanism on DM\u2010OS, thus laying a theoretical basis for the future application of BBR in implant restoration. Phenotypes were assessed in the DM rats after 4 w of gavage with BBR. Furthermore, BMSCs were cultured with high glucose and BBR. Cell Counting Kit\u20108, 2\u2032,7\u2032\u2010dichlorofluorescin diacetate (H<jats:sub>2<\/jats:sub>DCF\u2010DA), quantitative real\u2010time PCR (qRT\u2010PCR), and western blot were performed to estimate the cell proliferation, oxidative stress, and osteogenic differentiation. Moreover, the DM rats treated with BBR and insulin receptor substrate\u20101 anti\u2010sense oligonucleotide (IRS\u20101\u2010ASO) underwent a 4\u2010w implant\u2010healing period and then micro computed tomography (Micro\u2010CT) and histology were performed to verify the mechanism. Results showed that the 4\u2010w administration of BBR markedly improved the glucose metabolism and bone metabolism in the DM rats. in vitro experiments revealed that BBR alleviated high\u2010glucose\u2010inhibited osteogenesis of the BMSCs by upregulating reactive oxygen species (ROS)\u2010mediated IRS\u20101 signaling. Besides, injection of IRS\u20101\u2010ASO abolished the BBR promotion of implant osseointegration in the DM rats. In conclusion, targeting ROS\u2010mediated IRS\u20101 signaling, BBR acted as an efficient agent to advance osseointegration in DM, which indicated that BBR use is a good strategy for future implants restoration in diabetic patients.<\/jats:p>","DOI":"10.1002\/biof.1692","type":"journal-article","created":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T06:20:58Z","timestamp":1606285258000},"page":"80-92","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Berberine promotes peri\u2010implant osteogenesis in diabetic rats by <scp>ROS<\/scp>\u2010mediated <scp>IRS<\/scp>\u20101 pathway"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1423-4177","authenticated-orcid":false,"given":"Jingjing","family":"Shao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, 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