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An oxygen and glucose deprivation\/recovery (OGD\/R) model was established to evaluate the effects of mouse astrocytes (MA\u2010C) cell viability and apoptosis of stepwise exposure to oxygen and glucose deprivation followed by their replenishment. This assessment included using taurine upregulated gene 1\u2013small interfering RNAs (TUG1\u2010siRNA) transfection to determine the effects of TUG1 knockdown on MA\u2010C survival and apoptosis. Real\u2010time quantitative polymerase chain reaction (RT\u2010qPCR) was used to evaluate TUG1 and miR\u2010142\u20103p expression levels. The luciferase gene reporter assay was performed to validate that miR\u2010142\u20103p is a TUG1 target. Accordingly, the effects of miR\u2010142\u20103p knockdown on TUG1\u2010induced MA\u2010C apoptosis were determined using flow cytometry. Methyl thiazolyl tetrazolium (MTT) method was used to detect cell growth viability. Western blotting analysis was performed to detect the expression levels of apoptosis\u2010related proteins. TUG1 was upregulated, while miR\u2010142\u20103p was downregulated in the OGD\/R model of MA\u2010C cells. Inhibiting the expression of TUG1 could protect MA\u2010C cells and reverse the decrease in growth viability and increasing apoptosis of MA\u2010C cells caused by OGD\/R stimulation. On the other hand, the inhibition of miR\u2010142\u20103p offset the effect of TUG1 knockdown on cell viability and apoptosis. Inhibition of OGD\/R\u2010induced increases in TUG1 expression that in turn reduces miR\u2010142\u20103p upregulation may suppress reperfusion\u2010induced losses in cell viability.<\/jats:p>","DOI":"10.1002\/biof.1765","type":"journal-article","created":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T19:49:28Z","timestamp":1624304968000},"page":"819-827","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Knockdown of <scp>lncRNA TUG1<\/scp> attenuates cerebral ischemia\/reperfusion injury through regulating <scp>miR<\/scp>\u2010142\u20103p"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9609-8695","authenticated-orcid":false,"given":"Leibing","family":"Li","sequence":"first","affiliation":[{"name":"Department of Critical Care Medicine, People's Hospital of Rizhao  Rizhao 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