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It may lead to loss of proper tissue architecture and organ function in clinical diseases such as systemic sclerosis and liver fibrosis. Excess accumulation of collagens is considered the primary indicator of fibrosis. Notch signaling has been reported to be involved in the fibrosis of many different organs, including the liver. Our previous study showed that the uterine\u2010specific over\u2010activation of canonical Notch1 signaling in the mouse uterus (<jats:italic>Pgr<\/jats:italic><jats:sup><jats:italic>cre\/+<\/jats:italic><\/jats:sup> <jats:italic>Rosa26<\/jats:italic><jats:sup><jats:italic>N1ICD\/+<\/jats:italic><\/jats:sup>, OEx) results in complete infertility as a consequence of multiple developmental and physiological defects, together with increased collagen accumulation evidenced by Masson's staining. In this study, we further detected expressions of all 44 collagen genes in these Notch1 gain\u2010of\u2010function transgenic mice and found that 18 collagens have been largely affected. In another aspect, using an intrauterine adhesion model (IUA), we mimicked fibrosis in the mouse uterine. The results suggested that Notch receptors were upregulated only 3\u2009days after induction, and most of the fibril\u2010forming collagen began to upregulate 6\u2009days after the surgery. Furthermore, when induced IUA in the N1ICD\u2010OEx mice, the expression of collagens and fibrosis levels were significantly enhanced. At last, as a Notch signaling inhibitor, the \u03b3\u2010secretase inhibitor N\u2010[N\u2010(3,5\u2010difl uorophenacetyl)\u2010L\u2010alanyl]\u2010S\u2010phenylglycine t\u2010butyl ester (DAPT) pretreatment could alleviate the expression of collagens and the symptoms of fibrosis. These results demonstrate that Notch signaling may play a role in upregulating collagens expression in endometrial fibrosis and might be a potential target of fibrosis therapy in the endometrium.<\/jats:p>","DOI":"10.1002\/biof.1771","type":"journal-article","created":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T20:38:50Z","timestamp":1627504730000},"page":"852-864","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Notch1 signaling enhances collagen expression and fibrosis in mouse uterus"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4424-1645","authenticated-orcid":false,"given":"Qi\u2010Xin","family":"Xu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine South China Agricultural University  Guangzhou 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