{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:35:48Z","timestamp":1786977348824,"version":"build-2736575974"},"reference-count":87,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T00:00:00Z","timestamp":1633305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A number of approaches have been developed over the years to manage cancer, such as chemotherapy using low\u2010molecular\u2010mass molecules and radiotherapy. Here, enzymes can also find useful applications. Among them, oxidases have attracted attention because of their ability to produce reactive oxygen species (ROS, especially hydrogen peroxide) in tumors and potentially modulate the production of this cytotoxic compound when enzymes active on substrates present in low amounts are used, such as the\n                    <jats:sc>d<\/jats:sc>\n                    \u2010amino acid oxidase and\n                    <jats:sc>d<\/jats:sc>\n                    \u2010amino acid couple system. These treatments have been also developed for additional cancer treatment approaches, such as phototherapy, nutrient starvation, and metal\u2010induced hydroxyl radical production. In addition, to improve tumor specificity and decrease undesired side effects, oxidases have been targeted by means of nanotechnologies and protein engineering (i.e., by designing chimeric proteins able to accumulate in the tumor). The most recent advances obtained by using six different oxidases (i.e., the FAD\u2010containing enzymes glucose oxidase,\n                    <jats:sc>d<\/jats:sc>\n                    \u2010 and\n                    <jats:sc>l<\/jats:sc>\n                    \u2010amino acid oxidases, cholesterol oxidase and xanthine oxidase, and the copper\u2010containing amine oxidase) have been reported. Anticancer therapy based on oxidase\u2010based ROS production has now reached maturity and can be applied in the clinic.\n                  <\/jats:p>","DOI":"10.1002\/biof.1789","type":"journal-article","created":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T20:06:50Z","timestamp":1633550810000},"page":"384-399","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Reactive oxygen species as a double\u2010edged sword: The role of oxidative enzymes in antitumor therapy"],"prefix":"10.1002","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8384-7992","authenticated-orcid":false,"given":"Elena","family":"Rosini","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Life Sciences University of Insubria  Varese 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