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However, the underlying mechanisms of NH<jats:sub>3<\/jats:sub>\u2010induced tracheal cell autophagy remains poorly understood. In present study, chickens and LMH cells were used as NH<jats:sub>3<\/jats:sub> exposure models to investigate toxic effects. The change of tracheal tissues ultrastructure showed that NH<jats:sub>3<\/jats:sub> exposure induced autolysosomes. The differential expression of 12 circularRNAs (circRNAs) was induced by NH<jats:sub>3<\/jats:sub> exposure using circRNAs transcriptome analysis in broiler tracheas. We further found that circ\u2010IFNLR1 was down\u2010regulated, and miR\u20102188\u20105p was up\u2010regulated in tracheal tissues under NH<jats:sub>3<\/jats:sub> exposure. Bioinformatics analysis and dual luciferase reporter system showed that circ\u2010IFNLR1 bound directly to miR\u20102188\u20105p and regulated each other, and miR\u20102188\u20105p regulated RNF182. Overexpression of miR\u20102188\u20105p caused autophagy and its inhibition partially reversed autophagy in LMH cells which were caused by ammonia stimulation or knockdown of circ\u2010IFNLR1. The expressions of three autophagy\u2010related genes (LC3, Beclin 1, and BNIP3) were observably up\u2010regulated. Our results indicated that NH<jats:sub>3<\/jats:sub> exposure caused autophagy through circ\u2010IFNLR1\/miR\u20102188\u20105p\/RNF182. These results provided new insights for the study of ammonia on environmental toxicology on ceRNA and circRNAs in vivo and vitro.<\/jats:p>","DOI":"10.1002\/biof.1795","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T10:34:14Z","timestamp":1634726054000},"page":"416-427","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Ammonia induces autophagy via <scp>circ\u2010IFNLR1<\/scp>\/<scp>miR<\/scp>\u20102188\u20105p\/<scp>RNF182<\/scp> axis in tracheas of chickens"],"prefix":"10.1002","volume":"48","author":[{"given":"Tian\u2010yi","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Northeast Agricultural University  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northeast Agricultural University  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan\u2010ying","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northeast Agricultural University  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010chang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northeast Agricultural University  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7596-3632","authenticated-orcid":false,"given":"Meng\u2010yao","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northeast Agricultural University  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.343.6168.238"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2017.09.243"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.wasman.2019.11.022"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2019.113428"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.6b03694"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2017.12.103"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2019.110078"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2018.12.375"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2019.134035"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.140532"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2020.105056"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2020.111050"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.138686"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2019.06.235"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2018.11.188"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.736282"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2020.111231"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.145407"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2018.10.019"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.146591"},{"key":"e_1_2_11_22_1","article-title":"Calcium overload and reactive oxygen species accumulation induced by selenium deficiency promote autophagy in swine small intestine","volume":"486","author":"Zheng Y","year":"2021","journal-title":"Anim Nutr J"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/8460355"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2007.11.004"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.13583"},{"key":"e_1_2_11_26_1","doi-asserted-by":"crossref","unstructured":"C.Qianru H.Xueyuan Z.Bing Z.Qing Z.Kaixin L. 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