{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:31:05Z","timestamp":1786977065366,"version":"build-2736575974"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T00:00:00Z","timestamp":1683504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100022597","name":"Institute of Biophysics of the Czech Academy of Sciences","doi-asserted-by":"publisher","award":["68081707"],"award-info":[{"award-number":["68081707"]}],"id":[{"id":"10.13039\/501100022597","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["CZ.02.1.01\/0.0\/0.0\/16_026\/0008451"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_026\/0008451"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Peritoneal adhesions are postsurgical fibrotic complications connected to peritoneal inflammation. The exact mechanism of development is unknown; however, an important role is attributed to activated mesothelial cells (MCs) overproducing macromolecules of extracellular matrix (ECM), including hyaluronic acid (HA). It was suggested that endogenously\u2010produced HA contributes to the regulation of different fibrosis\u2010related pathologies. However, little is known about the role of altered HA production in peritoneal fibrosis. We focused on the consequences of the increased turnover of HA in the murine model of peritoneal adhesions. Changes of HA metabolism were observed in early phases of peritoneal adhesion development\n                    <jats:italic>in vivo<\/jats:italic>\n                    . To study the mechanism, human MCs MeT\u20105A and murine MCs isolated from the peritoneum of healthy mice were pro\u2010fibrotically activated by transforming growth factor \u03b2 (TGF\u03b2), and the production of HA was attenuated by two modulators of carbohydrate metabolism, 4\u2010methylumbelliferone (4\u2010MU) and 2\u2010deoxyglucose (2\u2010DG). The attenuation of HA production was mediated by upregulation of HAS2 and downregulation of HYAL2 and connected to the lower expression of pro\u2010fibrotic markers, including fibronectin and \u03b1\u2010smooth muscle actin (\u03b1SMA). Moreover, the inclination of MCs to form fibrotic clusters was also downregulated, particularly in 2\u2010DG\u2010treated cells. The effects of 2\u2010DG, but not 4\u2010MU, were connected to changes in cellular metabolism. Importantly, the inhibition of AKT phosphorylation was observed after the use of both HA production inhibitors. In summary, we identified endogenous HA as an important regulator of peritoneal fibrosis, not just a passive player during this pathological process.\n                  <\/jats:p>","DOI":"10.1002\/biof.1957","type":"journal-article","created":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T06:43:21Z","timestamp":1683528201000},"page":"940-955","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Endogenously produced hyaluronan contributes to the regulation of peritoneal adhesion development"],"prefix":"10.1002","volume":"49","author":[{"given":"Anna","family":"Kocurkova","sequence":"first","affiliation":[{"name":"Department of Experimental Biology, Faculty of Sciences Masaryk University  Brno Czech Republic"},{"name":"International Clinical Research Center St. Anne's University Hospital  Brno Czech Republic"},{"name":"Department of Biophysics of Immune System Institute of Biophysics of the Czech Academy of Sciences  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Kerberova","sequence":"additional","affiliation":[{"name":"Department of Biophysics of Immune System Institute of Biophysics of the Czech Academy of Sciences  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Nesporova","sequence":"additional","affiliation":[{"name":"Contipro a.s.  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