{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T09:59:13Z","timestamp":1792403953882,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T00:00:00Z","timestamp":1689811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1221098"],"award-info":[{"award-number":["1221098"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The synthesis rates of n\u20103 and n\u20106 polyunsaturated fatty acids (PUFAs) in rodents and humans are not agreed upon and depend on substrate availability independently of the capacity for synthesis. Therefore, we aimed to assess the activities of the enzymes for n\u20103 and n\u20106 PUFA synthesis pathways in liver, brain, testicle, kidney, heart, and lung, in relation to their protein concentration levels. Eight\u2010week\u2010old Balb\/c mice (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20098) were fed a standard chow diet (6.2% fat, 18.6% protein, and 44.2% carbohydrates) until 14\u2009weeks of age, anesthetized with isoflurane and tissue samples were collected (previously perfused) and stored at \u221280\u00b0C. The protein concentration of the enzymes (\u0394\u20106D, \u0394\u20105D, Elovl2, and Elovl5) were assessed by ELISA kits; their activities were assayed using specific PUFA precursors and measuring the respective PUFA products as fatty acid methyl esters by gas chromatographic analysis. The liver had the highest capacity for PUFA biosynthesis, with limited activity in the brain, testicles, and kidney, while we failed to detect activity in the heart and lung. The protein concentration and activity of the enzymes were significantly correlated. Furthermore, \u0394\u20106D, \u0394\u20105D, and Elovl2 have a higher affinity for n\u20103 PUFA precursors compared to n\u20106 PUFA. The capacity for PUFA synthesis in mice mainly resides in the liver, with enzymes having preference for n\u20103 PUFAs.\n                  <\/jats:p>","DOI":"10.1002\/biof.1992","type":"journal-article","created":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T04:54:45Z","timestamp":1689828885000},"page":"89-100","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":103,"title":["Protein concentrations and activities of fatty acid desaturase and elongase enzymes in liver, brain, testicle, and kidney from mice: Substrate dependency"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5055-3795","authenticated-orcid":false,"given":"Rodrigo","family":"Valenzuela","sequence":"first","affiliation":[{"name":"Nutrition Department, Faculty of Medicine University of Chile  Santiago Chile"},{"name":"Department of Nutritional Sciences, Temerty Faculty of Medicine University of Toronto  Toronto 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