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Thus, we tested Se deficiency in mice to determine its effects on intestinal bacterial balance and whether it induced liver injury. Serum Se concentration, lipopolysaccharide (LPS) level, and liver injury biomarkers were tested using a biochemical method, while pathological changes in the liver and jejunum were observed via hematoxylin and eosin stain, and a fluorescence spectrophotometer was used to evaluate intestinal permeability. Tight junction (TJ)\u2010related and toll\u2010like receptor (TLR) signaling\u2010related pathway genes and proteins were tested using quantitative polymerase chain reaction, western blotting, immunohistochemistry, and 16S ribosomal ribonucleic acid gene\u2010targeted sequencing of jejunum microorganisms. Se deficiency significantly decreased glutathione peroxidase activity and disrupted the intestinal flora, with the most significant effect being a decrease in <jats:italic>Lactobacillus reuteri<\/jats:italic>. The expression of TJ\u2010related genes and proteins decreased significantly with increased treatment time, whereas supplementation with Se, fecal microbiota transplantation, or <jats:italic>L. reuteri<\/jats:italic> reversed these decreases. Signs of liver injury and LPS content were significantly increased after intestinal flora imbalance or jejunum injury, and the levels of TLR signaling\u2010related genes were significantly increased. The results indicated that Se deficiency disrupted the microbiota balance, decreased the expression of intestinal TJ factors, and increased intestinal permeability. By contrast, LPS increased due to a bacterial imbalance, which may induce inflammatory liver injury via the TLR4 signaling pathway.<\/jats:p>","DOI":"10.1002\/biof.2006","type":"journal-article","created":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T14:57:35Z","timestamp":1694098655000},"page":"311-325","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Gut microbiota contribution to selenium deficiency\u2010induced gut\u2013liver inflammation"],"prefix":"10.1002","volume":"50","author":[{"given":"Guodong","family":"Wang","sequence":"first","affiliation":[{"name":"Henan Joint International Research Laboratory of Veterinary Biologics Research and Application Anyang Institute of Technology  Anyang Henan 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