{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T21:42:35Z","timestamp":1773351755997,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T00:00:00Z","timestamp":1703203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mesenchymal stem cells (MSCs) treatment has been widely explored as a therapy for myocardial infarction, peripheral ischemic vascular diseases, dilated cardiomyopathy, and pulmonary hypertension. Latest in vitro studies suggest that MSCs can differentiate into contractile cardiomyocytes. One of the best\u2010characterized MSCs products are MSCs\u2010derived extracellular vesicles (EVs). EVs are crucial paracrine effectors of MSCs. Based on previous works, paracrine effects of MSCs play a primary role in the regenerative ability. Hence, in the current paper, we focused our attention on an alternative approach, exploiting products derived from human dental pulp stem cells (hDPSCs) rather than MSCs themselves, which may denote a cost\u2010effective and safer approach. The focus has been on EVs and the bioactive molecules they contain to evaluate their ability to influence the differentiation process toward cardiomyogenic lineage. The expression of GATA4, ACTC1, CX43, and Nkx2.5 was evaluated using Immunofluorescence, real time\u2010PCR, and Western blotting analyses. Furthermore, the expression profiling analysis of the microRNA hsa\u2010miR\u2010200c\u20103p, targeting the <jats:italic>GATA4<\/jats:italic> gene, was studied. The hsa\u2010miR\u2010200c\u20103p was found significantly down\u2010regulated in both c\u2010hDPSCs + EVs\u2010hDPSCs and c\u2010hDPSCs + EVs\u2010HL\u20101 compared to untreated c\u2010hDPSCs underlying a possible epigenetic mechanism behind the prevalent up\u2010regulation of its targeted <jats:italic>GATA4<\/jats:italic> gene. The aim of the present work was to develop an in vitro model of hDPSCs able to differentiate into cardiomyocytes in order to investigate the role of EVs derived from hDPSCs and derived from HL\u20101 cardiomyocyte cell line in modulating the differentiation process toward cardiomyogenic lineage.<\/jats:p>","DOI":"10.1002\/biof.2025","type":"journal-article","created":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T09:19:27Z","timestamp":1703236767000},"page":"509-522","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Extracellular vesicles (<scp>EVs<\/scp>): A promising therapeutic tool in the heart tissue regeneration"],"prefix":"10.1002","volume":"50","author":[{"given":"Francesca","family":"Diomede","sequence":"first","affiliation":[{"name":"Department of Innovative Technologies in Medicine &amp; Dentistry University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Guarnieri","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Imaging and Clinical Sciences Center for Advanced Studies and Technology (CAST), University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Lanuti","sequence":"additional","affiliation":[{"name":"Department of Medicine and Aging Sciences Center for Advanced Studies and Technology (CAST), University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fani","family":"Konstantinidou","sequence":"additional","affiliation":[{"name":"Department of Psychological Health and Territorial Sciences School of Medicine and Health Sciences, \u201cG. d'Annunzio\u201d University of Chieti\u2010Pescara  Chieti Italy"},{"name":"Unit of Molecular Genetics Center for Advanced Studies and Technology (CAST), \u201cG. d'Annunzio\u201d University of Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Gatta","sequence":"additional","affiliation":[{"name":"Department of Psychological Health and Territorial Sciences School of Medicine and Health Sciences, \u201cG. d'Annunzio\u201d University of Chieti\u2010Pescara  Chieti Italy"},{"name":"Unit of Molecular Genetics Center for Advanced Studies and Technology (CAST), \u201cG. d'Annunzio\u201d University of Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thangavelu Soundara","family":"Rajan","sequence":"additional","affiliation":[{"name":"Research and Development Unit Theertha Biopharma Private limited, KIADB, Industrial Area  Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sante D.","family":"Pierdomenico","sequence":"additional","affiliation":[{"name":"Department of Innovative Technologies in Medicine &amp; Dentistry University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7459-4898","authenticated-orcid":false,"given":"Oriana","family":"Trubiani","sequence":"additional","affiliation":[{"name":"Department of Innovative Technologies in Medicine &amp; Dentistry University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7754-4653","authenticated-orcid":false,"given":"Guya Diletta","family":"Marconi","sequence":"additional","affiliation":[{"name":"Department of Innovative Technologies in Medicine &amp; Dentistry University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacopo","family":"Pizzicannella","sequence":"additional","affiliation":[{"name":"Department of Engineering and Geology University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Pescara Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5298"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.303614"},{"issue":"1","key":"e_1_2_9_4_1","first-page":"19","article-title":"Mesenchymal stem cell (MSCs) therapy for ischemic heart disease: a promising frontier. 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