{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T01:34:08Z","timestamp":1783388048541,"version":"3.54.6"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T00:00:00Z","timestamp":1703808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81620108026"],"award-info":[{"award-number":["81620108026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82273752"],"award-info":[{"award-number":["82273752"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82373700"],"award-info":[{"award-number":["82373700"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Kashin\u2013Beck disease (KBD) is an endemic, chronic degenerative joint disease in China. Exosomes miRNAs, as signaling molecules in intercellular communication, can transfer specific biological martials into target cell to regulate their function and might participate in the pathogenesis of KBD. We isolated serum and chondrocytes\u2010derived exosomes, miRNA sequencing revealed exosomes miRNA profiles and differentially expressed miRNAs (DE\u2010miRNAs) were identified. The target genes were predicted of known and novel DE\u2010miRNAs with TargetScan 5.0 and miRanda 3.3a database. Single\u2010cell RNA sequencing (scRNA\u2010seq) was performed to identify chondrocyte clusters and their gene signatures in KBD. And we performed comparative analysis between the serum and chondrocytes\u2010derived exosomes DE\u2010miRNA target genes and differentially expressed genes of each cell clusters. A total of 20 DE\u2010miRNAs were identified in serum\u2010derived exosomes. In the miRNA expression of chondrocytes\u2010derived exosomes, 53 DE\u2010miRNAs were identified. 16,063 predicted targets were identified as the target genes in the serum\u2010derived exosomes, 57,316 predicted targets were identified as the target genes in the chondrocytes\u2010derived exosomes. Seven clusters were labeled by cell type according to the expression of previously described markers. Three hundred fifteen common genes were found among serum\/chondrocytes\u2010derived exosomes DE\u2010miRNA target genes and DEGs identified by scRNA\u2010seq analysis. We firstly integratly analyzed the serum and chondrocytes exosomes miRNA with single\u2010cell RNA sequencing (scRNA\u2010seq) data of KBD chondrocyte, the results showed that DE\u2010miRNAs in exosomes might play a potential role in regulating genes expression in different KBD chondrocytes clusters by exosomes mediating cell\u2013cell communications functions, which could improve the new diagnosis and treatment methods for KBD.<\/jats:p>","DOI":"10.1002\/biof.2033","type":"journal-article","created":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T13:19:35Z","timestamp":1703855975000},"page":"725-737","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Integration of <scp>miRNA<\/scp> in exosomes and single\u2010cell <scp>RNA<\/scp>\u2010seq profiles in endemic osteoarthritis, <scp>Kashin\u2013Beck<\/scp> disease"],"prefix":"10.1002","volume":"50","author":[{"given":"Xi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Occupational and Environmental Health School of Public Health, Xi'an Jiaotong University Health Science Center  Xi'an Shaanxi China"},{"name":"School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases National Health and Family Planning Commission  Xi'an Shaanxi China"},{"name":"Key Laboratory for Disease Prevention and Control and Health Promotion of Shaanxi Province  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health School of Public Health, Xi'an Jiaotong University Health Science Center  Xi'an Shaanxi 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Province  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmou","family":"Zhao","sequence":"additional","affiliation":[{"name":"Foot and Ankle Surgery Department Honghui Hospital of Xi'an Jiaotong University  Shaanxi 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