{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T02:06:23Z","timestamp":1788573983558,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T00:00:00Z","timestamp":1716854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T00:00:00Z","timestamp":1716854400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006191","name":"Eski\u015fehir Osmangazi \u00dcniversitesi","doi-asserted-by":"publisher","award":["TOA\u20102022\u20102458"],"award-info":[{"award-number":["TOA\u20102022\u20102458"]}],"id":[{"id":"10.13039\/501100006191","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Inflammatory mediators that infiltrate the corneal stroma after corneal infections, trauma or refractive surgery can trigger the transformation of corneal keratocytes into myofibroblasts, resulting in highly irregular collagen deposition and subsequently corneal scarring. Mesenchymal stem cells (MSCs) can be used as therapeutic agents to regenerate corneal and conjunctival tissue damage, regulate inflammation, and reduce the development of limbal stem cell failure. The use of MSC\u2010derived exosomes as a cell\u2010free therapeutic vector is a novel therapeutic approach. This study aimed to assess the effect of exosomes obtained from melatonin (Mel)\u2010treated human limbal mesenchymal stem cells (hLMSCs) on na\u00efve hLMSCs and to determine their influence on the antifibrotic and pro\u2010regenerative pathways involved in corneal scarring. hLMSCs were treated with varying concentrations of Mel, followed by isolation and characterization of the procured exosomes (Mel\u2010prExos). These exosomes were added to the cell culture media of na\u00efve hLMSCs to examine their antifibrotic and pro\u2010regenerative effects. The expression of miR\u2010155, miR\u201029, TGF\u03b21, TGF\u03b23, PPAR\u03b3, and \u03b1\u2010SMA miRNAs and genes were compared between Mel\u2010treated hLMSCs and Mel\u2010prExo\u2010treated hLMSCs by using real\u2010time PCR. We found that at 1\u2009\u03bcM Mel and in the presence of Mel\u2010prExos, TGF\u03b21 was expressed 0.001\u2010fold, while TGF\u03b23 was expressed 0.6\u2010fold. miR\u201029 expression was increased 38\u2010fold in the control\u2010Exo group compared to that in the control group. Changes in TGF\u03b21\/\u03b23 and \u03b1\u2010SMA expression are associated with miR\u201029 and miR\u2010155. This approach could prove beneficial for ocular surface tissue engineering applications.<\/jats:p>","DOI":"10.1002\/biof.2085","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T07:19:56Z","timestamp":1716880796000},"page":"1287-1297","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Crosstalk among\n                    <scp>miR<\/scp>\n                    \u201029,\n                    <scp>\u03b1\u2010SMA<\/scp>\n                    , and\n                    <scp>TGF\u03b21<\/scp>\n                    \/\u03b23 in melatonin\u2010induced exosome (\n                    <scp>Mel\u2010prExo<\/scp>\n                    ) treated human limbal mesenchymal stem cells (\n                    <scp>hLMSCs<\/scp>\n                    ): An insight into scarless healing of the cornea"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4460-8467","authenticated-orcid":false,"given":"Burcugul","family":"Altug","sequence":"first","affiliation":[{"name":"Cellular Therapy and Stem Cell Production Application, Research Centre (ESTEM) Eskisehir Osmangazi University  Eskisehir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1231-9791","authenticated-orcid":false,"given":"Merve Nur","family":"Soykan","sequence":"additional","affiliation":[{"name":"Cellular Therapy and Stem Cell Production Application, Research Centre (ESTEM) Eskisehir Osmangazi University  Eskisehir T\u00fcrkiye"},{"name":"Department of Stem Cell Institute of Health Sciences, Eskisehir Osmangazi University  Eskisehir 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