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The aim of this research is to compare the potential effects of a viable probiotic (\n                    <jats:italic>Lactobacillus rhamnosus<\/jats:italic>\n                    GG) with those exerted by its heat\u2010inactivated paraprobiotic counterpart in a dietary rodent model of NAFLD. The probiotic administration effectively prevented the hepatic lipid accumulation induced by a high\u2010fat high\u2010fructose diet feeding, as demonstrated by chemical (lower TG content) and histological (lower steatosis grade and lobular inflammation) analyses. This effect was mainly mediated by the downregulation of lipid uptake (FATP2 protein expression) and upregulating liver TG release to bloodstream (MTTP activity) in rats receiving the probiotic. By contrast, the effect of the paraprobiotic preventing diet\u2010induced liver lipid accumulation was milder, and mainly derived from the downregulation of hepatic de novo lipogenesis (SREBP\u20101c protein expression and FAS activity) and TG assembly (DGAT2 and AQP9 protein expression). The obtained results demonstrate that under these experimental conditions, the effects induced by the administration of viable\n                    <jats:italic>L. rhamnosus<\/jats:italic>\n                    GG preventing liver lipid accumulation in rats fed a diet rich in saturated fat and fructose differ from those induced by its heat\u2010inactivated paraprobiotic counterpart.\n                  <\/jats:p>","DOI":"10.1002\/biof.2116","type":"journal-article","created":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T23:44:17Z","timestamp":1723506257000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Comparative effects of viable\n                    <scp>\n                      <i>Lactobacillus rhamnosus<\/i>\n                      GG\n                    <\/scp>\n                    and its heat\u2010inactivated paraprobiotic in the prevention of high\u2010fat high\u2010fructose diet\u2010induced\n                    <scp>non\u2010alcoholic fatty liver disease<\/scp>\n                    in rats"],"prefix":"10.1002","volume":"51","author":[{"given":"Laura Isabel","family":"Arellano\u2010Garc\u00eda","sequence":"first","affiliation":[{"name":"Nutrition and Obesity Group, Department of Pharmacy and Food Sciences, Faculty of Pharmacy and Lucio Lascaray Research Centre University of the Basque Country (UPV\/EHU)  Vitoria\u2010Gasteiz Spain"},{"name":"CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4312-9720","authenticated-orcid":false,"given":"I\u00f1aki","family":"Milton\u2010Laskibar","sequence":"additional","affiliation":[{"name":"Nutrition and Obesity Group, Department of Pharmacy and Food Sciences, Faculty of Pharmacy and Lucio Lascaray Research Centre University of the Basque Country (UPV\/EHU)  Vitoria\u2010Gasteiz Spain"},{"name":"CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III  Madrid Spain"},{"name":"BIOARABA Health Research Institute  Vitoria\u2010Gasteiz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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