{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:38:45Z","timestamp":1790980725112,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2008,12,16]],"date-time":"2008-12-16T00:00:00Z","timestamp":1229385600000},"content-version":"vor","delay-in-days":4002,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The role of vitamin E in cell regulation in addition to its function as an antioxidant has attracted attention. The effects of \u03b1\u2010tocopherol (T) and \u03b1\u2010tocopheryl succinate (TS) on transcriptional activation of the tumor necrosis factor alpha (TNF\u2010\u03b1 ) gene and nuclear factor kappa B (NF\u2010\u03ba B) activation were examined. Two stable transformants were used: TR\u20101 cells derived from THP\u20101 cells transfected with a vector contains the human TNF\u2010\u03b1 promoter (1.4\u2010kb) joined to the human placental alkaline phosphatase (PLAP) coding sequence, and B164 cells derived from the same cell line but carrying the vector containing the human \u03b2\u2010actin promoter (4.3\u2010kb) as a control.<\/jats:p><jats:p>The transfectants were cultured in the presence of TS, followed by stimulation with lipopolysaccharide (LPS). After stimulation, PLAP activity secreted into the culture medium was measured. TS reduced TNF\u2010\u03b1 transcriptional activity in a concentration\u2010dependent manner, while no effect was observed on that of the \u03b2\u2010actin promoter.<\/jats:p><jats:p>Gel shift assay revealed that THP\u20101 cells pretreated with TS and then with LPS showed inhibition of NF\u2010\u03ba B activity by 43% at 50 \u03bcM versus the TS\u2010untreated group. Since TS did not affect activator protein\u20101 (AP\u20101) activity under the same conditions, the inhibitory effect of TS on NF\u2010\u03ba B activation might be specific. However, T had no effect on the results of the gel shift assay.<\/jats:p><jats:p>Vitamin E transportation was analyzed by simultaneous determination of vitamin E and its derivatives using HPLC. The vitamin E recovered from culture pellets showed almost the same amounts of T and TS transferred and was recovered in unchanged form. These observations indicated that TS inhibited NF\u2010\u03ba B activation and\/or translocation to the nuclei in its unchanged form under the culture conditions used here.<\/jats:p><jats:p>These results suggested that vitamin E is involved in signal transduction via an effect distinct from its antioxidant function. To explain the lack of activity with T, it remains to be clarified whether physiological incorporation of T occurred.<\/jats:p>","DOI":"10.1002\/biof.5520070104","type":"journal-article","created":{"date-parts":[[2008,12,19]],"date-time":"2008-12-19T01:15:55Z","timestamp":1229649355000},"page":"21-30","source":"Crossref","is-referenced-by-count":53,"title":["Inhibition of NF\u2010<sub>\u03ba<\/sub>B transcriptional activity by \u03b1\u2010tocopheryl succinate"],"prefix":"10.1002","volume":"7","author":[{"given":"Tetsuya","family":"Nakamura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Goto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isao","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"1501S","DOI":"10.1093\/ajcn\/62.6.1501S","article-title":"Vitamin E: beyond antioxidant function","volume":"62","author":"Traber M.G.","year":"1995","journal-title":"Am. 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