{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:57:24Z","timestamp":1761101844085},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,12,16]],"date-time":"2008-12-16T00:00:00Z","timestamp":1229385600000},"content-version":"vor","delay-in-days":3637,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[1999,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dietary copper deficiency has been shown to significantly reduce acetylcholine (Ach)\u2010induced vascular smooth muscle relaxation. The current study was designed to examine the relative relationship between dietary copper and the vasodilator response to Ach in the microcirculation of the rat. Male weanling rats were fed a purified basal diet supplemented with 6.0, 3.0, 1.5 or 0.0 \u03bcg Cu\/g diet for 4 weeks to provide an adequate, two marginal, and deficient intakes of dietary copper. Arteriole dilation in response to increasing concentrations of acetylcholine (10<jats:sup>\u22121<\/jats:sup> to 10<jats:sup>\u22124<\/jats:sup> M) was measured in the in vivo cremaster muscle microcirculation for each dietary group. Liver copper and both aortic and erythrocyte Cu,Zn\u2010SOD activity were used as indices of systemic copper status. Dilation to the increasing concentrations of Ach was only different in the 0 \u03bcg Cu supplemented group compared to the copper\u2010adequate control values. However, the combined results showed an exponential increase in 10<jats:sup>\u22125<\/jats:sup> M Ach\u2010induced vasodilation as liver copper concentration increases from 0 \u03bcg Cu\/g dry wt. This relationship suggests that dilation is attenuated at liver Cu concentrations below 5 \u03bcg\/g dry wt. The results indicate that Ach\u2010induced vasodilation is copper\u2010dependent but that the pathway is not very sensitive to short\u2010term marginal restriction of copper intake.<\/jats:p>","DOI":"10.1002\/biof.5520100402","type":"journal-article","created":{"date-parts":[[2008,12,19]],"date-time":"2008-12-19T00:25:33Z","timestamp":1229646333000},"page":"321-327","source":"Crossref","is-referenced-by-count":12,"title":["Relationship between dietary copper concentration and acetylcholine\u2010induced vasodilation in the microcirculation of rats"],"prefix":"10.1002","volume":"10","author":[{"given":"D. A.","family":"Schuschke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. S.","family":"Percival","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. T.","family":"Saari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. N.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1093\/jn\/117.6.1085","article-title":"Effect of copper deficiency on erythrocyte membrane proteins in rats","volume":"117","author":"Johnson W. T.","year":"1987","journal-title":"Journal of Nutrition"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-203-43594"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.2307\/2683704"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.17.11.2975"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02783253"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.69.3.601"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-203-43576"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701144"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-200-43388"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/127.12.2274"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/jn\/122.7.1547","article-title":"Copper deficiency alters vasodilation in the rat cremaster muscle microcirculation","volume":"122","author":"Schuschke D. A.","year":"1992","journal-title":"Journal of Nutrition"},{"key":"e_1_2_1_13_2","unstructured":"D. A.Schuschke J. C.Falcone J. T.Saari J. T.Fleming S. A.Young H. W.SullivanandF. N.Miller Endothelial cell calcium mobilization to acetylcholine is attenuated in copper\u2010deficient rats. Unpublished (submitted to Endothelium)."},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.3109\/10739689509148281"},{"key":"e_1_2_1_15_2","first-page":"748","article-title":"Hemostatic mechanisms in marginally copper\u2010dependent rats","volume":"125","author":"Schuschke L. A.","year":"1995","journal-title":"Journal of Laboratory and Clinical Medicine"}],"container-title":["BioFactors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiof.5520100402","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biof.5520100402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T15:18:13Z","timestamp":1698506293000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/biof.5520100402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,1]]},"references-count":14,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1999,1]]}},"alternative-id":["10.1002\/biof.5520100402"],"URL":"https:\/\/doi.org\/10.1002\/biof.5520100402","archive":["Portico"],"relation":{},"ISSN":["0951-6433","1872-8081"],"issn-type":[{"value":"0951-6433","type":"print"},{"value":"1872-8081","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,1]]}}}