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The purpose of this study was to investigate the relationship between dietary copper and carcinogen administration on PKC isozyme accumulation and aberrant crypt foci (ACF) formation in rats fed 0.9 and 7.7 \u03bcg Cu\/g diet. After 24 and 31 d on the diets, the rats were injected with either dimethylhydrazine (DMH) (25 mg\/kg i.p.) or saline and killed at two time points (2 wk and 8 wk after DMH). Rats fed low dietary copper had significantly lower (<jats:italic>p<\/jats:italic>&lt;0.0001) hematocrits, hemoglobin, ceruloplasmin activity and plasma and liver copper concentrations than rats fed adequate dietary copper. Ingestion of low dietary copper significantly (<jats:italic>p<\/jats:italic>&lt;0.005) increased the formation of DMH\u2010induced ACF (116.8 vs 59.6). Low dietary copper significantly (<jats:italic>p<\/jats:italic>&lt;0.05) decreased the concentration of PKC \u03b1, \u03b4, and \u03b6 in the colon at 2 wk but not at 8 wk. Thus, changes in PKC isoform protein concentration may be related to increased susceptibility of copper\u2010deficient animals to colon cancer.<\/jats:p>","DOI":"10.1002\/biof.5520150102","type":"journal-article","created":{"date-parts":[[2008,12,19]],"date-time":"2008-12-19T00:31:46Z","timestamp":1229646706000},"page":"11-26","source":"Crossref","is-referenced-by-count":11,"title":["Dietary copper and dimethylhydrazine affect protein kinase C isozyme protein and mRNA expression and the formation of aberrant crypts in colon of rats"],"prefix":"10.1002","volume":"15","author":[{"given":"Cindy D.","family":"Davis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. 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