{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T16:19:29Z","timestamp":1793377169905,"version":"4.5.0"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":36,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioFactors"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Daidzin (DZN) is a natural flavonoid compound derived from soybeans that has recently been recognized for its potential antitumor properties. In traditional Chinese medicine, soybeans and their extracts are extensively used to prevent and treat various diseases. To evaluate the therapeutic efficacy of DZN on human glioblastoma through in\u00a0vivo and in\u00a0vitro experiments, and through multi\u2010omics analyses to elucidate potential molecular mechanisms. Cell viability of LN\u2010229 and U\u201087MG glioblastoma cells was assessed using the CCK\u20108 assay. Protein and mRNA levels of proliferation and apoptosis\u2010related genes were analyzed via Western blotting and qPCR. Metabolomics and transcriptomics identified key pathways and targets, which were confirmed by in\u2010cell Western blotting and expression correlation analysis. The in\u00a0vivo antitumor effects of DZN were evaluated in nude mice with LN\u2010229 tumors. DZN treatment reduced cell viability, migration, and survival in a dose\u2010dependent manner, demonstrating strong antitumor effects in both in\u00a0vitro and in\u00a0vivo models. Multi\u2010omics analysis identified amino acid metabolism and ubiquitin\u2010mediated proteolysis as key mechanisms. Bioinformatics highlighted LRP5 as a prognostic biomarker in glioblastoma. DZN decreased LRP5 activity, downregulated p\u2010GSK\u20103<jats:italic>\u03b2<\/jats:italic>, and promoted c\u2010Myc degradation, thereby inhibiting the Wnt signaling pathway. In\u00a0vivo, DZN significantly reduced tumor size and Ki67 expression. These findings highlight LRP5 as a promising therapeutic target, with DZN emerging as a potent LRP5 inhibitor and exhibiting significant antitumor effects in glioblastoma.<\/jats:p>","DOI":"10.1002\/biof.70025","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T09:37:18Z","timestamp":1749202638000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Natural Product Daidzin Inhibits Glioma Development via Suppressing the <scp>LRP5<\/scp>\u2010Mediated <scp>GSK<\/scp>\u20103<i>\u03b2<\/i>\/c\u2010Myc Signaling Pathway"],"prefix":"10.1002","volume":"51","author":[{"given":"Yijing","family":"Pan","sequence":"first","affiliation":[{"name":"Hubei Provincial Engineering Technology Research Center for Chinese Medicine Processing School of Pharmacy, Hubei University of Chinese Medicine  Wuhan 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