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Oxidative stress induces inflammation and myonecrosis in early disease stages in both mdx mice and human patients with Duchenne muscular dystrophy (DMD). This study aimed to investigate the effects of hydroethanolic soy extract (HSE) on dystrophic muscle in mdx mice (a mouse model of DMD), based on the estrogenic action of total soy isoflavones in reducing oxidative stress and inflammation. Phytochemical profiling of HSE was performed by UPLC\u2010PDA\u2010MS, and in silico molecular docking identified putative targets of the main isoflavones. Fourteen\u2010day\u2010old male mdx mice were treated orally with HSE (57\u2009mg\/kg\/day, ~2\u2009mg\/kg genistein) for 45\u2009days. Muscle function, biochemical markers, histopathology, and molecular indicators of inflammation and oxidative stress were evaluated. HSE contained multiple isoflavone classes, showing strong binding affinities for monoamine oxidase and estrogen receptors. The isoflavone\u2010rich HSE showed no toxic effects in mdx mice and demonstrated a clear protective influence on skeletal muscle pathology. Treatment with HSE led to improved muscle integrity, as evidenced by increased muscle strength and a marked reduction in markers of muscle damage such as IgG\u2010positive fibers and serum CK levels. Additionally, HSE effectively mitigated the inflammatory response, with significant downregulation of NF\u2010\u03baB, reduction of inflammatory and macrophage infiltration areas, and attenuation of oxidative stress markers. The antioxidant response was enhanced, reflected by lower ROS production, decreased lipid peroxidation (4\u2010HNE and lipofuscin), and elevated levels of endogenous antioxidants (catalase and GSH). Moreover, HSE appeared to support muscle regeneration by increasing the proportion of centrally nucleated fibers while modulating myogenic regulatory pathways, as indicated by decreased MyoD expression. Isoflavone\u2010rich HSE safely improved muscle integrity and function in mdx mice by attenuating oxidative stress and inflammation through modulation of the ROS\/NF\u2010\u03baB pathway. These findings highlight the therapeutic potential of soy\u2010derived isoflavones as a complementary approach for managing DMD.<\/jats:p>","DOI":"10.1002\/biof.70062","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T03:05:10Z","timestamp":1764385510000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Hydroethanolic Soy Extract Rich in Isoflavones Protects Dystrophic Mutant Mice From Oxidative Stress and Inflammation via the\n                    <scp>ROS<\/scp>\n                    \/\n                    <scp>NF<\/scp>\n                    \u2010\n                    <scp>\u03baB<\/scp>\n                    Feedback Pathway"],"prefix":"10.1002","volume":"51","author":[{"given":"Beatriz Godinho","family":"Nascimento","sequence":"first","affiliation":[{"name":"Department of Anatomy Institute of Biomedical Sciences, Federal University of Alfenas (UNIFAL\u2010MG)  Alfenas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"La\u00eds Leite","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Anatomy Institute of Biomedical Sciences, Federal University of Alfenas (UNIFAL\u2010MG)  Alfenas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wagner","family":"Corsini","sequence":"additional","affiliation":[{"name":"Department of Anatomy Institute of Biomedical Sciences, Federal University of Alfenas (UNIFAL\u2010MG)  Alfenas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina Fiuza","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Anatomy Institute of Biomedical Sciences, Federal University of Alfenas (UNIFAL\u2010MG)  Alfenas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via Pavani","family":"Teodoro","sequence":"additional","affiliation":[{"name":"Department of Anatomy Institute of Biomedical Sciences, Federal University of Alfenas (UNIFAL\u2010MG)  Alfenas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice Gabrielle Marques","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Anatomy Institute of Biomedical Sciences, Federal University of Alfenas (UNIFAL\u2010MG)  Alfenas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wagner","family":"de Melo Fischer Macedo","sequence":"additional","affiliation":[{"name":"Department of Food and Drugs, Faculty of Pharmaceutical Sciences Federal University of Alfenas (UNIFAL\u2010MG)  Alfenas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1763-5697","authenticated-orcid":false,"given":"Maur\u00edcio Ariel","family":"Rostagno","sequence":"additional","affiliation":[{"name":"Multidisciplinary Laboratory of Food and Health (LabMAS), School of Applied Sciences (FCA) University of Campinas (UNICAMP)  Limeira Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Jose Dias","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Food and Drugs, Faculty of Pharmaceutical Sciences Federal University of Alfenas (UNIFAL\u2010MG)  Alfenas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Vinicius Expedito","family":"de Souza","sequence":"additional","affiliation":[{"name":"Human Genetics Laboratory Natural Sciences Institute, Federal University of Alfenas (UNIFAL\u2010MG)  Alfenas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Marcos","family":"Oliveira\u2010Silva","sequence":"additional","affiliation":[{"name":"Human Genetics Laboratory 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