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Finding new therapeutic targets could help to develop treatments that attenuate its severity. The present study investigated the immunomodulatory potential of bioaccessible sulforaphane (SFN, 0.100\u2009\u03bcg\/mL) from broccoli by\u2010products. Interestingly, the main results evidenced that at this physiological concentration, SFN contributed to reducing the secretion of pro\u2010inflammatory interleukins (IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    , IL\u20106, IL\u201017, IL\u201018, IL\u201023, TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    ) by intestinal epithelium up to ~56%, whereas enhancing anti\u2010inflammatory cytokines (IL\u201010, IL\u20104, IL\u201013) between ~24% and ~71%. These changes adjusted the proportion of CD86\n                    <jats:sup>+<\/jats:sup>\n                    and CD206\n                    <jats:sup>+<\/jats:sup>\n                    during macrophage differentiation, associated with the prevention of immune\u2010mediated IBD. In addition, a reduction in the expression of macrophage\u2010dependent pro\u2010inflammatory cytokines and an augmentation of the tolerogenic classes were observed. The combined use of intestinal epithelial (Caco\u20102) and monocytic (THP\u20101) cell lines established an in\u00a0vitro model of the epithelium\u2010macrophage crosstalk, thereby enhancing the physiological relevance of our findings. These results were confirmed using a pure SFN\u2010based model system, which demonstrated SFN's contribution to the anti\u2010inflammatory properties of broccoli stalk and bridged the gap between in\u00a0vitro findings and potential dietary\/therapeutic applications. Thereby, this work demonstrated that dietary SFN contributes to a large extent to the reshaping capacity of the phytochemical burden of broccoli stalks, on the interleukin profile secreted by epithelium and macrophages, as well as the macrophage differentiation, thus supporting the valorisation of broccoli by\u2010products for preventing and managing inflammatory conditions, such as IBD.\n                  <\/jats:p>","DOI":"10.1002\/biof.70101","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T04:45:31Z","timestamp":1776141931000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioaccessible Sulforaphane Drives Macrophage Migration and Differentiation by Reprogramming the Interleukin Profile in Intestinal Inflammation"],"prefix":"10.1002","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7231-6480","authenticated-orcid":false,"given":"Sonia","family":"Medina","sequence":"first","affiliation":[{"name":"Laboratorio de Fitoqu\u00edmica y Alimentos Saludables (LabFAS), CSIC, CEBAS Campus Universitario de Espinardo  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6358-3694","authenticated-orcid":false,"given":"Concepci\u00f3n","family":"Medrano\u2010Padial","sequence":"additional","affiliation":[{"name":"Laboratorio de Fitoqu\u00edmica y Alimentos Saludables (LabFAS), CSIC, CEBAS Campus Universitario de Espinardo  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Garc\u00eda\u2010Viguera","sequence":"additional","affiliation":[{"name":"Laboratorio de Fitoqu\u00edmica y Alimentos Saludables (LabFAS), CSIC, CEBAS Campus Universitario de Espinardo  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6232-712X","authenticated-orcid":false,"given":"Ra\u00fal","family":"Dom\u00ednguez\u2010Perles","sequence":"additional","affiliation":[{"name":"Laboratorio de Fitoqu\u00edmica y Alimentos Saludables (LabFAS), CSIC, CEBAS Campus Universitario de Espinardo  Murcia 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