{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T09:40:46Z","timestamp":1778924446106,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2006,11,24]],"date-time":"2006-11-24T00:00:00Z","timestamp":1164326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to investigate the frequency, location and type of rpoB mutations in <jats:italic>Mycobacterium tuberculosis<\/jats:italic> isolated from patients in Belarus. Tuberculosis cases are increasing every year in Belarus. Moreover, resistance to anti\u2010tuberculosis drugs, especially to rifampicine, has increased. In this study, 44 rifampicine\u2010resistance <jats:italic>M. tuberculosis<\/jats:italic> clinical isolates (including multidrug\u2010resistant isolates) were subjected to DNA sequencing analysis of the hypervariable region (hot\u2010spot) of the rpoB gene originating from different geographical regions in Belarus. Sixteen different types of mutations were identified. The most common point mutations were in codons 510 (47.7%), 526 (45.5%), 523 (40.86%) and 531 (29.5%). Eleven isolates (27.7%) carried one mutation and 23 (52%), 7 (16%), 3 (7%) of isolates carried 2, 3 and 4 mutations, respectively. A characteristic, prominent finding of this study was high frequency of multiple mutations in different codons of the rpoB gene (27.7%) and also the detection of unusual types of mutations in the 510 codon, comprising CAG mutations (deletion or changing, to CTG, CAC or CAT). In our study, the change TTG in codon 531 was found in 92% of isolates and the change TGC in 8% of isolates. A TAC change in codon 526 was not found, but the GAC change was found in all isolates. Isolates of <jats:italic>M. tuberculosis<\/jats:italic> isolated in Belarus were characterized by the wide spectrum of the important mutations and might belong to the epidemic widespread clones.<\/jats:p>","DOI":"10.1002\/biot.200600153","type":"journal-article","created":{"date-parts":[[2006,11,24]],"date-time":"2006-11-24T05:49:01Z","timestamp":1164347341000},"page":"1447-1452","source":"Crossref","is-referenced-by-count":8,"title":["Molecular characterization of rpoB gene mutations in rifampicine\u2010resistant <i>Mycobacterium tuberculosis<\/i> isolates from tuberculosis patients in Belarus"],"prefix":"10.1002","volume":"1","author":[{"given":"Leonid P.","family":"Titov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saeed","family":"Zakerbostanabad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veranika","family":"Slizen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larisa","family":"Surkova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Taghikhani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmadreza","family":"Bahrmand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,12,7]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.7.2509-2512.2002"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1585-1589.2004"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1128\/AAC.42.7.1853","article-title":"Contribution of rpoB mutations to development of Rifampycin cross\u2010resistance in Mycobacterium tuberculosis.","volume":"42","author":"Williams D. L.","year":"1998","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_2_1_5_2","unstructured":"Titov L. P. Problems of new and recently appeared infections of present time. International scientific conference Minsk Belarus May 26\u201027 1997 4\u201010 (in Russian)."},{"key":"e_1_2_1_6_2","unstructured":"Titov L. P. Kluchenovich V. I. Microorganism resistance to antimicrobials and disinfectant agents in Belarus International scientific conference Minsk Belarus May 27\u201028 2003 4\u201011 (in Russian)."},{"key":"e_1_2_1_7_2","unstructured":"Gurevich G. L. Borshevsky V. V. Skriagina E. M. Ostrovko A. P. Multidrug resistant tuberculosis: its frequency and clinical and epidemiological importance. 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