{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T10:32:01Z","timestamp":1761561121082,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,1,16]],"date-time":"2007-01-16T00:00:00Z","timestamp":1168905600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recombinant rabies virus glycoprotein (rRVGP) was expressed in <jats:italic>Drosophila melanogaster<\/jats:italic> Schneider 2 (S2) cells. The cDNA encoding the entire <jats:italic>RVGP<\/jats:italic> gene was cloned in an expression plasmid under the control of the constitutive actin promoter (Ac), which was co\u2010transfected into S2 cells together with a hygromycin selection plasmid. Selected S2 cell populations (S2AcRVGP) had a decreased ability to grow and consume substrates, when compared to the non\u2010transfected cells (S2). They were shown, by PCR, to express the <jats:italic>RVGP<\/jats:italic> gene and mRNA and, by immunoblotting, to synthesize the rRVGP in its expected molecular mass of 65 kDa. ELISA kinetic studies showed the rRVGP expression in cell lysates and supernatants attaining concentrations of 300 \u03bcg\/L. By flow cytometry analysis, about 30% of the cells in the co\u2010transfected populations were shown to express the rRVGP. Cell populations selected by limiting dilution expressed higher rRVGP yields. Mice immunized with rRVGP were shown to synthesize antibodies against rabies virus and be protected against experimental infection with rabies virus. The data presented here show that S2 cells can be suitable hosts for the rRVGP expression, allowing its synthesis in a high degree of physical and biological integrity.<\/jats:p>","DOI":"10.1002\/biot.200600211","type":"journal-article","created":{"date-parts":[[2007,1,16]],"date-time":"2007-01-16T11:51:08Z","timestamp":1168948268000},"page":"102-109","source":"Crossref","is-referenced-by-count":42,"title":["Rabies virus glycoprotein expression in Drosophila S2 cells. I. Functional recombinant protein in stable co\u2010transfected cell line"],"prefix":"10.1002","volume":"2","author":[{"given":"Adriana Y.","family":"Yokomizo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soraia A. C.","family":"Jorge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato M.","family":"Astray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orlando G.","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise S. P. Q.","family":"Horton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldo","family":"Tonso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noel","family":"Tordo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Augusto","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15792-9"},{"key":"e_1_2_1_3_2","unstructured":"Pastoret P. P. Brochier B. Aguilar\u2010Setin A. Blancou J. Vaccination against rabies in: Pastoret P. P. Blancou J. Vanner P. Verschueren C. (Eds.).Veterinary Vaccinology Elsevier Amsterdam 1997 p 616."},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.8.2.115"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.218"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10917"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.09.016"},{"key":"e_1_2_1_8_2","first-page":"207","article-title":"Development of rabies DNA vaccine using a recombinant plasmid.","volume":"49","author":"Rai N.","year":"2005","journal-title":"Acta Virol."},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004490050630"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2005.05.002"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"3742","DOI":"10.1128\/jvi.71.5.3742-3750.1997","article-title":"Folding of rabies virus glycoprotein: Epitope acquisition and interaction with endoplasmic reticulum chaperones.","volume":"71","author":"Gaudin Y.","year":"1997","journal-title":"J. Virol."},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(02)00267-8"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81063-0"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.22.7194"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(85)90224-5"},{"key":"e_1_2_1_16_2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1128\/JVI.73.1.225-233.1999","article-title":"Chimeric lyssavirus glycoproteins with increased immunological potential.","volume":"73","author":"Jallet C.","year":"1999","journal-title":"J. Virol."},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.02.039"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(88)90179-X"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90551-5"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1235"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0958-1669(91)90038-7"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-003-1223-9"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_24_2","unstructured":"Perrin P. Lafon M. Sureau P. Enzyme linked immuno\u2010sorbent assay (ELISA) for the determination of glycoprotein content of rabies vaccines in: Meslin F. X Kaplan M. M Koprowaski H (Eds) Laboratory Techniques in Rabies WHO Geneva 1996 pp. 383\u2010388."},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(98)00357-0"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1702(94)90076-0"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1702(92)90095-Q"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00449-005-0033-0"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050413"},{"key":"e_1_2_1_30_2","doi-asserted-by":"crossref","first-page":"4957","DOI":"10.1128\/jvi.69.8.4957-4962.1995","article-title":"Cross\u2010protection of mice against a global spectrum of rabies virus variants.","volume":"69","author":"Lodmell D. L.","year":"1995","journal-title":"J. Virol."}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiot.200600211","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.200600211","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T14:41:52Z","timestamp":1759934512000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.200600211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1002\/biot.200600211"],"URL":"https:\/\/doi.org\/10.1002\/biot.200600211","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"type":"print","value":"1860-6768"},{"type":"electronic","value":"1860-7314"}],"subject":[],"published":{"date-parts":[[2007,1]]}}}