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In the present work whole\u2010cell reduction of pentoxifylline [1\u2010(5\u2010oxohexyl)\u20103,5\u2010dimethylxanthine] to LSF [1\u2010(5R\u2010hydroxyhexyl)\u20103,5\u2010dimethylxanthine] using Lactobacillus kefiri DSM 20587 was investigated. Glucose or 2\u2010propanol was used as a co\u2010substrate to regenerate the NADPH cofactor. The reaction conditions were optimized. The influence of different concentrations of co\u2010substrates on the yield and enantioselectivity of the biotransformation of pentoxifylline into LSF were tested. Maximum yield (100%) of biotransformation was reached in the presence of glucose as a co\u2010substrate. At glucose concentrations of 675 and 900 mM the bioreduction of pentoxifylline proceeded highly enantioselectively (enantiomeric excess for the <jats:italic>R<\/jats:italic> enantiomer of 98%).<\/jats:p>","DOI":"10.1002\/biot.200600236","type":"journal-article","created":{"date-parts":[[2007,2,7]],"date-time":"2007-02-07T06:31:49Z","timestamp":1170829909000},"page":"492-496","source":"Crossref","is-referenced-by-count":10,"title":["Enantioselective reduction of pentoxifylline to lisofylline using whole\u2010cell Lactobacillus kefiri biotransformation"],"prefix":"10.1002","volume":"2","author":[{"given":"El\u017cbieta","family":"P\u0119kala;","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Godawska\u2010Matysik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorota","family":"\u017belaszczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,4,4]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/chir.10129"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(01)00266-X"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(01)01009-2"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2004.02.005"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/tcr.20016"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","unstructured":"Kula M.\u2010T. 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