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In this work, the substitution of fetal bovine serum by yeastolate and a soy hydrolysate (Hy Soy) for the culture of <jats:italic>Drosophila melanogaster<\/jats:italic> Schneider 2 cells transfected for the production of rabies virus G glycoprotein was evaluated. TC100 supplemented with glucose, glutamine, lipid emulsion and Pluronic F68 was employed as basal medium. Results show that yeastolate was more efficient on cell growth stimulation than Hy Soy. Cells adapted in medium formulation supplemented with 3 g\/L yeastolate, 1% lipid emulsion, 10 g\/L glucose, 3.5 g\/L glutamine and 0.1% Pluronic F68 attained a maximum concentration of 10.7 \u00d7 10<jats:sup>6<\/jats:sup> cells\/mL, with the expression of 9.4 ng\/mL G glycoprotein.<\/jats:p>","DOI":"10.1002\/biot.200700048","type":"journal-article","created":{"date-parts":[[2007,6,21]],"date-time":"2007-06-21T05:17:48Z","timestamp":1182403068000},"page":"1399-1407","source":"Crossref","is-referenced-by-count":14,"title":["Culture of transgenic <i>Drosophila melanogaster<\/i> Schneider 2 cells in serum\u2010free media based on TC100 basal medium"],"prefix":"10.1002","volume":"2","author":[{"given":"Adriana L. 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