{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T01:12:27Z","timestamp":1760404347424,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2008,6,19]],"date-time":"2008-06-19T00:00:00Z","timestamp":1213833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Suspension microsphere immunoassays are rapidly gaining recognition in antigen identification and infectious disease biodetection due to their simplicity, versatility and high\u2010throughput multiplex screening. We demonstrate a multiplex assay based on antibody\u2010functionalized barcoded resins (BCRs) to identify pathogen antigens in complex biological fluids. The binding event of a particular antibody on given bead (fluorescence) and the identification of the specific pathogen agent (vibrational fingerprint of the bead) can be achieved in a dispersive Raman system by exciting the sample with two different laser lines. Anthrax protective antigen, <jats:italic>Franciscella tularensis<\/jats:italic> lipopolysaccharide and CD14 antigens were accurately identified and quantified in tetraplex assays with a detection limit of 1 ng\/mL. The rapid, versatile and simple analysis enabled by the BCRs demonstrates their potential for multiplex antigen detection and identification in a reconfigurable microarray format.<\/jats:p>","DOI":"10.1002\/biot.200700236","type":"journal-article","created":{"date-parts":[[2008,6,19]],"date-time":"2008-06-19T08:16:28Z","timestamp":1213863388000},"page":"948-953","source":"Crossref","is-referenced-by-count":13,"title":["Multiplex pathogen detection based on spatially addressable microarrays of barcoded resins"],"prefix":"10.1002","volume":"3","author":[{"given":"David R.","family":"Blais","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramon A.","family":"Alvarez\u2010Puebla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan P.","family":"Bravo\u2010Vasquez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hicham","family":"Fenniri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John Paul","family":"Pezacki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,7,15]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1201-1271"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2006.06.036"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406159101"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05498"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac049318q"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605889103"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2007.08.065"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2007.06.035"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/cc0600831"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1134929"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja035665q"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja016375h"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3773(20001215)39:24<4483::AID-ANIE4483>3.0.CO;2-4"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/cc050128i"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2007.03.014"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/la700701x"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1203\/01.pdr.0000199907.61549.94"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0543"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1039\/b615477h"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2007.05.065"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc0498020"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2255804"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/biot.200600213"},{"key":"e_1_2_1_25_2","doi-asserted-by":"crossref","first-page":"4141","DOI":"10.1021\/ac0701031","article-title":"Surface\u2010enhanced Raman scattering immunoassays using a rotated capture substrate.","volume":"79","author":"Driskell J. 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