{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:50:05Z","timestamp":1759971005087,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,3,18]],"date-time":"2009-03-18T00:00:00Z","timestamp":1237334400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This report describes the immunogenicity and protective efficacy of <jats:italic>Escherichia coli<\/jats:italic>\u2010expressed recombinant protective antigen (rPA) in New Zealand White rabbits and Rhesus Macaques against an aerosol challenge with <jats:italic>Bacillus anthracis<\/jats:italic> spores (IVRI strain, tox+cap+). A dose\u2010ranging study was performed in which it became evident that the level of anti\u2010PA IgG and toxin\u2010neutralizing antibody titer was directly proportional to the dose of rPA administered. However, the onset time of primary and secondary immune response was not dependent on the dosage. Revaccination of primed animals with the same threshold dose yielded a robust and rapid secondary response. Quantitative differences in peak titers were obtained for both the animal models, in addition to qualitative differences in the immune kinetics. In spite of a weak priming response, the secondary response in rabbits peaked earlier than that in macaques once the booster dose was administered. However, evaluation of the post\u2010challenge quantitative anti\u2010rPA ELISA titer measurements indicated higher titers for non\u2010human primates as compared to the lagomorphs. Importantly, 100% protection was seen for the dosage groups that received \u226525 \u03bcg rPA, following a challenge against a target dose of 1000 LD<jats:sub>50<\/jats:sub> of aerosolized spores of <jats:italic>Bacillus anthracis.<\/jats:italic><\/jats:p>","DOI":"10.1002\/biot.200800213","type":"journal-article","created":{"date-parts":[[2009,3,18]],"date-time":"2009-03-18T03:25:36Z","timestamp":1237346736000},"page":"391-399","source":"Crossref","is-referenced-by-count":14,"title":["Efficacy of recombinant anthrax vaccine against <i>Bacillus anthracis<\/i> aerosol spore challenge: Preclinical evaluation in rabbits and Rhesus monkeys"],"prefix":"10.1002","volume":"4","author":[{"given":"Anil","family":"Chawla","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuchi","family":"Midha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rakesh","family":"Bhatnagar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,3,18]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01137.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.55.1.647"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0706.010604"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.281.18.1735"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1128\/jb.85.1.230-236.1963","article-title":"Studies on immunity in anthrax. 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