{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:15:09Z","timestamp":1787238909597,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,6,25]],"date-time":"2009-06-25T00:00:00Z","timestamp":1245888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Human BFK (BCL\u20102 family kin) is a novel pro\u2010apoptotic BCL\u20102 family member specifically expressed in the gastrointestinal tract. BFK has the characteristic BH3 domain, which was shown to be essential for the apoptosis\u2010inducing activity of pro\u2010apoptotic BCL\u20102 family members. When overexpressed, BFK interacts with BCL\u2010XL and BCL\u2010W but not BCL\u20102 or BAD in co\u2010immunoprecipitations studies. We find that BFK exhibits striking similarity to BID in the way it is activated through cleavage during apoptosis. The endogenous and cleaved versions of BFK are readily recognized by the rabbit and mouse sera raised against human BFK. An ideal caspase 3 or 7 target sequence, DEVD (amino acids 38\u201341), is evident N\u2010terminal to the BH3 domain. A recombinant version of the protein containing all residues downstream of the putative caspase cleavage site induces apoptosis in human colon cancer cells, HCT116, and in wild\u2010type mouse embryonic fibroblasts (MEFs), which can be reversed by co\u2010expression of BCL\u2010XL or BCL\u2010W. BFK becomes activated through caspase\u2010dependent cleavage during DNA damage\u2010induced apoptosis. The cleaved form of the protein is dependent on the presence of BAX or BAK for its ability to induce apoptosis, since BAX\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    \u2010BAK\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    double\u2010knockout MEFs are completely resistant to BFK\u2010induced apoptosis.\n                  <\/jats:p>","DOI":"10.1002\/biot.200900091","type":"journal-article","created":{"date-parts":[[2009,6,25]],"date-time":"2009-06-25T08:59:01Z","timestamp":1245920341000},"page":"1046-1054","source":"Crossref","is-referenced-by-count":9,"title":["A putative role for human BFK in DNA damage\u2010induced apoptosis"],"prefix":"10.1002","volume":"4","author":[{"given":"Nesrin","family":"\u00d6z\u00f6ren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naohiro","family":"Inohara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"N\u00fa\u00f1ez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,7,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.9.2.701"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5302.967"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2005.06.012"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1304105"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.12.030"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401204"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.05.050"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-0018"},{"key":"e_1_2_1_10_2","first-page":"7316","article-title":"Tissue\u2010specific induction of p53 targets in vivo.","volume":"62","author":"Fei P.","year":"2002","journal-title":"Cancer Res."},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90412-3"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5468.1053"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1103603"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00214-3"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb866"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.8.4386"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90508-N"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.20.10717"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.7.1686"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1126903"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.150082"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiot.200900091","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.200900091","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T11:53:20Z","timestamp":1759924400000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.200900091"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,7]]},"references-count":20,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2009,7]]}},"alternative-id":["10.1002\/biot.200900091"],"URL":"https:\/\/doi.org\/10.1002\/biot.200900091","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"value":"1860-6768","type":"print"},{"value":"1860-7314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,7]]}}}