{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T01:11:26Z","timestamp":1759972286123,"version":"build-2065373602"},"reference-count":16,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2009,10,12]],"date-time":"2009-10-12T00:00:00Z","timestamp":1255305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to achieve expression of recombinant rabies virus glycoprotein (rRVGP) in <jats:italic>Drosophila<\/jats:italic> S2 cells. For this, a cDNA coding for the selection hygromycin antibiotic and the cDNA encoding the RVGP protein under the control of the constitutive actin promoter (Ac) were cloned in an expression plasmid, which was transfected into S2 cells. S2 cell populations (S2AcRVGPHy) showed rRVGP expression in cell lysates, attaining concentrations up to 1.5 \u03bcg\/10<jats:sup>7<\/jats:sup> cells (705 \u03bcg\/L). Of the transfected cells, 20% were shown to express the rRVGP. Cell subpopulations selected by limiting dilution expressed higher rRVGP yields and 90% of the cells were shown to express the rRVGP. Cell populations re\u2010selected by addition of hygromycin were shown to express 10 times higher rRVGP yields. The data presented here show that <jats:italic>Drosophila<\/jats:italic> S2 cells can be efficiently transfected with an expression\/selection plasmid for rRVGP expression, allowing its synthesis with a high degree of physical and biological integrity. The importance of subpopulation selection was indicated by the increasing rRVGP yields during these procedures.<\/jats:p>","DOI":"10.1002\/biot.200900123","type":"journal-article","created":{"date-parts":[[2009,10,12]],"date-time":"2009-10-12T14:45:36Z","timestamp":1255358736000},"page":"1578-1581","source":"Crossref","is-referenced-by-count":3,"title":["Rabies virus glycoprotein expression in <i>Drosophila<\/i> S2 cells: Influence of re\u2010selection on protein expression"],"prefix":"10.1002","volume":"4","author":[{"given":"Alexandra Souza","family":"dos Santos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Alexandre Nobre","family":"Lemos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Augusto","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soraia Attie Calil","family":"Jorge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,11,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15792-9"},{"key":"e_1_2_1_3_2","unstructured":"Pastoret P. P. Brochier B. Aguilar\u2010Setin A. Blancou J. Vaccination against rabies in: Pastoret P. P. Blancou J. Vanner P. Verschueren C. (Eds.) Veterinary Vaccinology Elsevier Amsterdam 1997 p. 616."},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2005.05.002"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.22.7194"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.02.039"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-70-1-133"},{"key":"e_1_2_1_8_2","first-page":"263","article-title":"The rabies virus genome: An overview.","volume":"60","author":"Tordo N.","year":"1993","journal-title":"Onderstepoort J. Vet. Res."},{"key":"e_1_2_1_9_2","first-page":"353","article-title":"Cell lines derived from late embryonic stages of Drosophila melanogaster.","volume":"27","author":"Schneider I.","year":"1972","journal-title":"J. Embryol. Exp. 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Laboratory techniques in rabies World Health Organization Geneva 1996 pp. 383\u2013388."},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-007-9060-9"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-008-9154-z"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-008-9129-0"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.10.3015"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.10.3010"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiot.200900123","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.200900123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T20:48:43Z","timestamp":1759956523000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.200900123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11]]},"references-count":16,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2009,11]]}},"alternative-id":["10.1002\/biot.200900123"],"URL":"https:\/\/doi.org\/10.1002\/biot.200900123","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"type":"print","value":"1860-6768"},{"type":"electronic","value":"1860-7314"}],"subject":[],"published":{"date-parts":[[2009,11]]}}}