{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T20:45:55Z","timestamp":1791146755652,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,3,26]],"date-time":"2010-03-26T00:00:00Z","timestamp":1269561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The immobilization conditions of commercial ketoreductase for continuous enantioselective reduction in the gas\u2010phase reaction were investigated with respect to the immobilization efficiency (residual activity and protein loading) and the gas\u2010phase reaction efficiency (initial reaction rate, half\u2010life, and enantioselectivity). For the analyses, ketoreductase was first immobilized by physical deposition on glass supports and the reduction of 2\u2010butanone to (<jats:italic>S<\/jats:italic>)\u20102\u2010butanol with the concomitant regeneration of NADH by 2\u2010propanol was used as a model reaction. The optimal conditions of enzyme immobilization were obtained using an absolute pressure of 100 hPa for drying, a pH between 6.5 and 7.0, and a buffer concentration of 50 mM. The buffer concentration in particular had a strong effect on both the enzyme activity and enantioselectivity. Under optimal immobilization conditions, the thermostability of ketoreductase in the gas\u2010phase system was enhanced compared to the aqueous\u2010phase system, while the enantioselectivity was successfully maintained at a level identical to that of the native enzyme. These results indicate that the gas\u2010phase reaction has a great potential for industrial production of chiral compounds, but requires careful optimization of immobilization conditions for the reaction to progress effectively.<\/jats:p>","DOI":"10.1002\/biot.200900287","type":"journal-article","created":{"date-parts":[[2010,3,26]],"date-time":"2010-03-26T14:15:11Z","timestamp":1269612911000},"page":"520-525","source":"Crossref","is-referenced-by-count":15,"title":["Immobilization conditions of ketoreductase on enantioselective reduction in a gas\u2010solid bioreactor"],"prefix":"10.1002","volume":"5","author":[{"given":"Kazuhito","family":"Nagayama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antje C.","family":"Spiess","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jochen","family":"B\u00fcchs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,5,3]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260450503"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1039\/b405869k"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19960320)49:6<709::AID-BIT13>3.0.CO;2-0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/bp034334e"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-005-0169-5"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/bp050316g"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01020822"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260340908"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260420515"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcatb.2004.09.010"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200700095"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BFb0103304"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/b98910"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1263\/jbb.99.340"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(92)90298-L"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1006\/jcis.1997.4895"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.10.3192"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(79)90176-1"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiot.200900287","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.200900287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T12:37:52Z","timestamp":1759927072000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.200900287"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["10.1002\/biot.200900287"],"URL":"https:\/\/doi.org\/10.1002\/biot.200900287","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"value":"1860-6768","type":"print"},{"value":"1860-7314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5]]}}}