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Here we examine four antibiotics, hygromycin B, neomycin, puromycin, and Zeocin\u2122, and their corresponding selector genes, using a green fluorescent protein (GFP) as a reporter in two model cell lines, HT1080 and HEK293. We identify Zeocin\u2122 as the best selection agent for cell line development in human cells. In comparison to the other selection systems, Zeocin\u2122 is able to identify populations with higher fluorescence levels, which in turn leads to the isolation of better clonal populations and less false positives. Furthermore, Zeocin\u2122\u2010resistant populations exhibit better transgene stability in the absence of selection pressure compared to other selection agents. All isolated Zeocin\u2122\u2010resistant clones, regardless of cell type, exhibited GFP expression. By comparison, only 79% of hygromycin B\u2010resistant, 47% of neomycin\u2010resistant, and 14% of puromycin\u2010resistant clones expressed GFP. Based on these results, we rank Zeocin\u2122 &gt; hygromycin B \u223c puromycin &gt; neomycin for cell line development in human cells. Furthermore, this study demonstrates that selection marker choice does indeed impact cell line development.<\/jats:p>","DOI":"10.1002\/biot.201200364","type":"journal-article","created":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T14:27:35Z","timestamp":1362148055000},"page":"811-821","source":"Crossref","is-referenced-by-count":48,"title":["Evaluating the influence of selection markers on obtaining selected pools and stable cell lines in human cells"],"prefix":"10.1002","volume":"8","author":[{"given":"Amanda M.","family":"Lanza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do Soon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hal 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