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Several lipases used in industrial processes, although stable in the presence of other organic solvents, are inactivated by methanol at or below the concentration optimal for biodiesel production, making it necessary to use stepwise methanol feeding or pre\u2010treatment of the enzyme. In this review article we focus on what is currently know about methanol inactivation of lipases, a phenomenon which is not common to all lipase enzymes, with the goal of improving the biocatalytic process. We suggest that different mechanisms can lead to inactivation of different lipases, in particular substrate inhibition and protein unfolding. Attempts to improve the performances of methanol sensitive lipases by mutagenesis as well as process engineering approaches are also summarized<\/jats:p>","DOI":"10.1002\/biot.201400158","type":"journal-article","created":{"date-parts":[[2014,7,21]],"date-time":"2014-07-21T06:28:03Z","timestamp":1405924083000},"page":"22-30","source":"Crossref","is-referenced-by-count":168,"title":["Effects of methanol on lipases: Molecular, kinetic and process issues in the production of biodiesel"],"prefix":"10.1002","volume":"10","author":[{"given":"Marina","family":"Lotti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Pleiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Valero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pau","family":"Ferrer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,21]]},"reference":[{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/07388550902823674"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22256"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ejlt.200900078"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2010.05.012"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apenergy.2014.01.017"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11746-005-1076-3"},{"key":"e_1_2_9_8_2","doi-asserted-by":"crossref","unstructured":"Uphues G. 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