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Adenovirus and poxvirus vectors are among the viral vectors that are most frequently used to develop prophylactic vaccines against infectious diseases as well as therapeutic cancer vaccines. This mini\u2010review describes the trends and processes in large\u2010scale production of adenovirus and poxvirus vectors to meet the needs of clinical applications. We briefly describe the general principles for the production and purification of adenovirus and poxvirus viral vectors. Currently, adenovirus and poxvirus vector manufacturing methods rely on well\u2010established cell culture technologies. Several improvements have been evaluated to increase the yield and to reduce the overall manufacturing cost, such as cultivation at high cell densities and continuous downstream processing. Additionally, advancements in vector characterization will greatly facilitate the development of novel vectored vaccine candidates.<\/jats:p>","DOI":"10.1002\/biot.201400390","type":"journal-article","created":{"date-parts":[[2015,4,24]],"date-time":"2015-04-24T04:30:33Z","timestamp":1429849833000},"page":"741-747","source":"Crossref","is-referenced-by-count":43,"title":["Large\u2010scale adenovirus and poxvirus\u2010vectored vaccine manufacturing to enable clinical trials"],"prefix":"10.1002","volume":"10","author":[{"given":"H\u00e9la","family":"Kallel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amine A.","family":"Kamen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,24]]},"reference":[{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-009-1400-1"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00005"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.10.004"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2011.03.006"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1204186"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2013.12.029"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.04.029"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.10.095"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00439"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.13.15"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2009.01141.x"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.07.035"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.05.048"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3009185"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/v6083055"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.2010.519332"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.09.1"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2013.235"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.03.021"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.21.11349"},{"key":"e_1_2_9_22_2","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1089\/aid.1992.8.1445","article-title":"Highly attenuated poxvirus vectors.","volume":"8","author":"Tartaglia J.","year":"1992","journal-title":"AIDS Res. 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