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They present a range of surface antigens in a native conformation and have natural properties like immunogenicity, self\u2010adjuvation and uptake by immune cells which make them attractive for application as vaccines against pathogenic bacteria. In particular with\n                    <jats:italic>Neisseria meningitidis<\/jats:italic>\n                    , they have been investigated extensively and an OMV\u2010containing meningococcal vaccine has recently been approved by regulatory agencies. Genetic engineering of the OMV\u2010producing bacteria can be used to improve and expand their usefulness as vaccines. Recent work on meningitis B vaccines shows that OMVs can be modified, such as for lipopolysaccharide reactogenicity, to yield an OMV product that is safe and effective. The overexpression of crucial antigens or simultaneous expression of multiple antigenic variants as well as the expression of heterologous antigens enable expansion of their range of applications. 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