{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:03:55Z","timestamp":1792782235181,"version":"4.5.0"},"reference-count":98,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,2,18]],"date-time":"2015-02-18T00:00:00Z","timestamp":1424217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"European 195 Community\u00e2\u20ac\u2122s Seventh Framework Programme","award":["FP7 VECTORIE project number 261466"],"award-info":[{"award-number":["FP7 VECTORIE project number 261466"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Arthropod\u2010borne arboviruses form a continuous threat to human and animal health, but few arboviral vaccines are currently available. Advances in expression technology for complex, enveloped virus\u2010like particles (eVLPs) create new opportunities to develop potent vaccines against pathogenic arboviruses. In this short review, I highlight the successes and challenges in eVLP production for members of the three major arbovirus families:\n                    <jats:italic>Flaviviridae<\/jats:italic>\n                    (e.g., dengue, West Nile, Japanese encephalitis);\n                    <jats:italic>Bunyaviridae<\/jats:italic>\n                    (e.g., Rift Valley fever); and\n                    <jats:italic>Togaviridae<\/jats:italic>\n                    (e.g., chikungunya). The results from pre\u2010clinical testing will be discussed as well as specific constraints to the large\u2010scale manufacture and purification of eVLPs, which are complex assemblies of membranes and viral glycoproteins. Insect cells emerge as ideal substrates for correct arboviral glycoprotein folding and posttranslational modification to yield high quality eVLPs. Furthermore, baculovirus expression in insect cell culture is scalable and has a proven safety record in industrial human and veterinary vaccine manufacturing. In conclusion, eVLPs produced in insect cells using modern biotechnology have a realistic potential to be used in novel vaccines against arboviral diseases.\n                  <\/jats:p>","DOI":"10.1002\/biot.201400427","type":"journal-article","created":{"date-parts":[[2015,2,18]],"date-time":"2015-02-18T08:56:51Z","timestamp":1424249811000},"page":"659-670","source":"Crossref","is-referenced-by-count":38,"title":["Enveloped virus\u2010like particles as vaccines against pathogenic arboviruses"],"prefix":"10.1002","volume":"10","author":[{"given":"Gorben P.","family":"Pijlman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2009.10.008"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-46115-6_3"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.139"},{"key":"e_1_2_6_5_2","first-page":"874345","article-title":"Zoonoses and one health: A review of the literature.","volume":"2014","author":"Bidaisee S.","year":"2014","journal-title":"Parasitol. 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